超临界CO_2萃取技术分离和纯化苦丁茶中熊果酸的工艺  

Supercritical Fluid CO_2 Extraction and Purification of Urosolic Acid from Ilex kudingcha C.J.Tseng

作　　者：李宏杨[1,2] 刘飞[3] 张凤琴[3] 李小龙[3] 刘国民[1,2] 

机构地区：[1]海南大学热带生物资源教育部国家重点实验室,海南海口570228 [2]海南大学苦丁茶研究所,海南海口570228 [3]湖南工业大学绿色包装与生物纳米技术应用湖南省重点实验室,湖南株洲412007

出　　处：《热带生物学报》2012年第3期258-260,270,共4页Journal of Tropical Biology

基　　金：国家科技支撑计划子课题(2007BAD76B05-02)

摘　　要：对超临界CO2萃取苦丁茶中熊果酸的工艺进行了研究,并采用酸碱沉淀法纯化萃取液。结果表明:在以无水乙醇作为夹带剂,CO2流量为20L·h-1,萃取温度为45℃,萃取压力为30MPa,萃取时间为3h的萃取条件下,萃取效果最好,萃取率可达0.453%,最终能得到w=21.44%纯度的熊果酸粗品。该工艺优于传统的熊果酸提取方法,适于产业化生产。Ursolic acid was extracted supercritically from llex kudingcha C. J. Tseng with fluid CO2. The extract liquid was purified with acid and alkali and detected with high performance liquid chromatography （HPLC）. Optimal extracting conditions resulted to be absolute ethanol as entrainer, CO2 flow rate at 20 L·h-1, extracting temperature at 45 ℃, extracting pressure at 30 MPa and extracting time for 3 h, and the extraction rate was upto 0.453%. Moreover, crude products of ursolic acid with a purity of 21.44% were produced finally. This extrac- tion technology is superior to the traditional solvent extraction of ursolic acid, and suitable for industrial produc- tion.

关 键 词：超临界CO2 萃取技术 苦丁茶 熊果酸 

分 类 号：R284.1[医药卫生—中药学]