花叶艳山姜茎尖离体快繁技术  

Rapid propagation of Alpinia zerumbet cv. vaniegata

作　　者：潘梅[1] 符瑞侃 王景飞[1] 吕德任 黄赛[1] 

机构地区：[1]海南省农业科学院园林花卉研究所,海南海口571100

出　　处：《热带生物学报》2012年第3期267-270,共4页Journal of Tropical Biology

摘　　要：以花叶艳山姜(Alpinia zerumbet cv.vaniegata)的茎尖作为外植体,研究了花叶艳山姜的离体快繁技术。结果表明:花叶艳山姜茎尖接种在MS+BA3.0mg·L-1+NAA0.2mg·L-1+白糖30g·L-1的培养基上,30d后长出幼芽;丛生芽在MS+6-BA2.0～2.5mg·L-1+NAA0.2mg·L-1+白糖30g·L-1的培养基上,增殖效果最佳,增殖倍数达4.0,芽生长正常,且色泽浓绿;芽苗在1/2MS+NAA1.0～1.5mg·L-1+白糖30g·L-1的培养基上,生根效果较好,根系粗壮,生根率为93.0%;组培苗移栽在V河沙:V珍珠岩:V表土=1:1:1的混合基质中,成活率可达70.1%。Shoot tips were used as explants to mass propagate Alpinia zerumbet cv. vaniegata. The shoot tips were cultured on the MS medium at the presence of BA 3.0 mg · L-1 ＋ NAA 0.2 mg · L- 1 ＋ sugar 30 mg·L- 1 for 30 days and produced adventitious shoots continuously. These shoots were transferred onto the MS medium sup- plemented with 6 - BA 2.0--2.5 mg · L-1 ＋ NAA 0.2 mg · L- 1 ＋ sugar 30 mg · L-1 for further culture and were well proliferated with a proliferation coefficient of 4.0. The multiplied shoots grew normal with dark green color and were rooted well with stout roots on the 1/2MS medium supplemented with NAA 1.0--1.5 mg · L-1 with a rooting rate of 93.0%. The rooted plantlets of A. zerumbet cv. vaniegata were transplanted in the mixed substrate （ river sand： perlite： surface soil = 1 ： 1 ： 1 ）, and survived upto 70.1%.

关 键 词：花叶艳山姜 茎尖 快速繁殖 

分 类 号：S567.2[农业科学—中草药栽培]