不同序列Cp G ODN对牙周膜细胞的促增殖作用  

Proliferative effect of Cp G ODN with different sequences on human periodental ligament cells

作　　者：秦雁雁[1] 申玉芹[1] 任春霞[1] 郭恪[1] 林崇韬[1] 王丽颖[2] 于永利[3] 

机构地区：[1]吉林大学口腔医院牙周病科,吉林长春130021 [2]吉林大学白求恩医学院分子生物学教研室,吉林长春130021 [3]吉林大学白求恩医学院免疫学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2012年第5期876-879,I0001,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(201115105)

摘　　要：目的:用不同序列Cp G ODN刺激牙周膜细胞(PDLCs),筛选对PDLC有促增殖作用的Cp G ODN序列,为Cp G ODN在牙周病治疗上的应用提供实验依据。方法:收集因正畸治疗需要而拔除的健康前磨牙48颗,年龄10～15岁。利用组织块原代培养法培养人PDLCs,取第4和5代细胞用于实验。实验组用1～12号Cp G ODN刺激PDLCs,分别培养24、48和72h,用PBS做对照组,MTT比色法检测吸光度A492值。结果:原代培养的PDLCs呈长梭形或星形,胞突细长,中央有圆形或椭圆型的胞核,抗波形丝蛋白染色阳性,抗角蛋白染色阴性,为中胚层来源细胞。与对照组比较,实验组Cp G ODN的A均值升高,其中9、11号Cp G ODN与对照组比较A值明显升高(P<0.05或P<0.01)。结论:Cp G ODN有促人PDLCs增殖作用,其中9、11号Cp G ODN可显著促进人PDLCs增殖。Objective To screen the optimal Cp G ODN for inducing the proliferation of human periodental ligament cells（PDLCs）,and to provide the experimental basis for the application of Cp G ODN in treatment of periodentitis.Methods The 48 healthy premolars of young people（10-15 years old） undergoing tooth extraction for orthodontic treatment were collected.Tissue explant primary culture was used to cultivate the PDLCs in vitro.The cells from the 4th to 5th passages were selected for experiment.1-12 Cp G ODN were respectively given to PDLCs for 24,48,and 72 h,and the cells treated with PBS only were used as control group.MTT assay was used to detect the cell proliferation.The above experiments were repeated for 3 times.Results The human PDLCs of primary culture were obtained with tissue cultivation.The primary cultivated PDLCs showed long spindle appearance.By immunocytochemical analysis,these cells were positive to antibodies against vimentin,and negative to antibodies against ceratin,indicating themselves to be mesoderm-derived fibroblasts.The mean A values in Cp G ODN groups were all higher than those in PBS control group,and the A values of the 9th and 11th Cp G ODNs were significantly higher than that in PBS control group（P0.05 or P0.01）.Conclusion Cp G ODN can promote the proliferation of human PDLCs,and the 9th and 11th Cp G ODN can significantly promote the proliferation of human PDLCs.

关 键 词：牙周膜细胞 CP G ODN 细胞增殖 

分 类 号：R392.11[医药卫生—免疫学]