HPLC法测定贵州不同产地牛膝中齐墩果酸含量  

Determination of Oleanolic acid in Achyranthes bidentata From Different Cultivation by HPLC

作　　者：丁秦[1] 杨玉琴[1] 朱京祎 张丽艳[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]贵州省贵阳市第二人民医院,贵州贵阳550023

出　　处：《中国民族民间医药》2012年第22期40-41,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：贵州省中医药管理局资助项目[黔中医药科发(2007)3号]

摘　　要：目的:比较贵州不同产地牛膝中齐墩果酸含量差异,为牛膝质量标准的建立提供参考。方法:采用高效液相色谱法,色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm);流动相:乙腈-0.01%磷酸水溶液(90∶10);检测波长λ=204 nm;流速1.0 mL.min-1。结果:贵州不同产地牛膝中齐墩果酸含量差异较大,含量介于:1.05%～3.05%。结论:该法方法操作简便,结果稳定可靠,可用于牛膝的质量评价。Objective：To determine content of oleanolic acid in Achyranthes bidentata from the various cultivation areas of Guizhou.Method：A Diamonsil C18 colum（250 mm×4.6 mm,5 μm） was used with 0.01% phosphoric acid aqueous solution acetonitrile（10：90） as the mobile phase.The detective wavelength was set at 204 nm and the flow rate was 1.0 ml·min-1.Result：Different regions in Guizhou the content of oleanolic acid in Achyranthes bidentata are difference.The content of oleanolic acid ranged from 1.05%~3.05%.Conclusion：The method was simple,reproducible and reliable.It can be used to control the quality of Achyranthes bidentata.

关 键 词：牛膝 齐墩果酸 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]