早花及晚花灰毡毛忍冬中绿原酸含量分析  

Analysis on the Content of Chlorogenic Acid from Early-flowering and Later-flowering Lonicera Macranthoides

作　　者：余正文[1,2] 刘方方[1] 何磊磊[1] 玉秋萍[1] 张妮[1] 宋庆发[1,2] 乙引[1,2] 

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550001 [2]贵州师范大学贵州省植物生理与发育重点实验室,贵州贵阳550001

出　　处：《现代食品科技》2012年第11期1572-1574,1580,共4页Modern Food Science and Technology

基　　金：国家自然科学基金(31060056);贵州师范大学博士科研项目(11904-05032110014);贵州省中药现代化专项(黔科合中药字[2011]5046号);贵州省科技基金项目(黔科合J字[2011]2368号);贵州省科技创新人才团队建设项目(黔科合人才团队[2009]4007号)

摘　　要：研究早花和晚花灰毡毛忍冬花蕾及叶中绿原酸含量，筛选适宜贵州北部推广种植优良品种，采用超声波辅助提取灰毡毛忍冬中的绿原酸，高效液相色谱法测定其含量，色谱条件为：Diamonsil柱（5μm，C18（2），250mm×4．6mm），甲醇／乙腈（5：11）-0．1％磷酸=20：80，流速1．0mL／min，柱温40℃，检测波长325nm。结果表明旱花品种花蕾中绿原酸含量为2．36％～3．44％，叶中绿原酸含量为0．49％o～1．11％；晚花花蕾中绿原酸含量为2．60％～4．07％，叶中绿原酸含量为1．92％．2．83％。因此晚花衣毡毛忍冬是一种富绿原酸育种系。To explore suitable planting fine Lonicera macranthoides varieties in north Guizhou, the content of chlorogcnic acid in flower buds and leaves of early-flowering and later-flowering varieties were researched. Chlorogenic acid were obtained with ultrasonnic wave-assisted extraction technique, and analyzed using HPLC methods with operating condition as foUows： Diamonsil （5 μm, C18（2）, 250 mm×4.6 mm）,the volume flow 1.0mL/min, the column temperature 40℃ and the detection wavelength 325nm, The mobile phase methanol/acetonitrile （5：11）-0.1% phosphoric acid=20：80. The result showed that the content of chlorogenic acid ranged from 2.36% to 3.44% in flower buds and from 0.49% to 1.11% in leaves of early-flowering Lonicera macranthoides. In flower buds and leaves of later-flowering one, it as in the range of 2.60%,～4.07% and 1.92%～2.83 %, respectively. It was concluded that the later-flowering Lonicera macranthoide was a rich-ehlomgenic acid breeding sWairL

关 键 词：灰毡毛忍冬 早花 晚花 绿原酸 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]