木薯产业化组培育苗技术研究  

Study on Industrialization Tissue Culture Seedlings Technology of Cassava

作　　者：时群 何贵整 陈乃明 陈丽文 梁小娟 杨利平 

机构地区：[1]钦州市林业科学研究所,广西钦州535000

出　　处：《黑龙江农业科学》2012年第11期25-27,共3页Heilongjiang Agricultural Sciences

基　　金：钦州市科学研究与技术开发计划资助项目(20123602)

摘　　要：以"NE199"和"钦州本地"木薯优良品种为材料,进行木薯产业化组培育苗技术研究。结果表明:组培微型扦插培养基使用MS+BA 0.06～0.08mg.L-1+NAA 0.02mg.L-1+GA30.02mg.L-1,培养光照强度1 500～3 000 1x,培养温度25～30℃,繁殖系数可达4.0～4.2,腋芽萌芽率达90%以上,玻璃化率和叶片黄化率可控制在2%和6%以内,移栽基质为黄心土∶河沙=8∶1,移栽成活率可达90%以上,可以实现木薯组培育苗产业化生产。Taking ＇NE199＇ and ＇the Qinzhou local＇ cassava as experiment materials to study industrialization tissue culture seedlings technology.The results showed that：The micro-cuttage medium using MS＋BA 0.06~0.08 mg·L-1＋NAA 0.02 mg·L-1＋GA3 0.02 mg·L-1,light intensity of 1 500~3 000 1x and incubation temperature of 25~30℃,the propagation coefficient could up to 4.0~4.2,axillary bud sprouting rate could reach above 90%,the vitrification rate and leaf yellowing rate could be controlled within 2% and 6%.With the cultivation loess∶sand=8∶1,the transplanting survival rate could reach more than 90%,which could achieve the industrialization of cassava plant tissue culture.

关 键 词：木薯 组织培养 微型扦插 产业化 

分 类 号：S533[农业科学—作物学]