土壤中双氰胺降解及与降解菌的关系  

Degradation of Dicyandiamide in Soil and Its Relationship with Degrading Microorganism

作　　者：罗涛[1] 王煌平[1] 张晓玲[2] 张青[1] 蔡开地 翁伯琦[4] 

机构地区：[1]福建省农业科学院土壤肥料研究所,福州350013 [2]福建农林大学资环学院,福州350002 [3]福建省莆田市荔城区土壤肥料技术站,福建莆田351131 [4]福建省农业科学院农业生态研究所,福州350013

出　　处：《农业环境科学学报》2012年第11期2174-2179,共6页Journal of Agro-Environment Science

基　　金：国家科技支撑计划(2012BAD14B15);福建省财政专项-福建省农业科学院科技创新团队建设基金(STIF-Y01);福建省省属公益类科研院所基本科研专项(2011R1024-5)

摘　　要：采用灭菌土培、非灭菌土培、田间蔬菜种植、添加外源降解菌土培试验研究了土壤中双氰胺(DCD)降解及与降解菌的关系。结果显示,不论是单施DCD、尿素配施DCD,还是碳酸氢铵配施DCD的土壤,灭菌处理的土壤中DCD半衰期分别比不灭菌处理的长13.56、5.79、14.51d。降解菌生长期间,降解菌总量(x)与DCD降解呈显著正相关,拟合的线性方程为y=3.1841x-2.5452,r=0.9752。外源DCD降解真菌可在灭菌土壤中定殖并有效降解DCD,培养15d后,U+DCD+DCD降解菌处理土壤中DCD降解真菌的数量增加至36.40×105cfu,且DCD含量极显著降低。这些结果表明土壤中DCD降解与降解菌关系极为密切,添加外源真菌加速了土壤中DCD降解。Sterilized,non-sterilized and application of exogenous fungi with soil cultivations,and field plating tests were employed to investigate the degradation of dicyandiamide（DCD）,relating to soil microorganism populations.Application of DCD（control）,DCD with urea,or DCD with ammonium bicarbonate in sterilized soil cultivations,the half-lives of DCD degradation were longer than those of non-sterilized soils of 13.56 d,5.79 d and 14.51 d,respectively.The sigmoidal growth of microbial populations（x）observed significantly correlation with the degradation of DCD（y）,showing the linear equation of y=3.184 1x-2.545 2,r=0.975 2.Application of exogenous fungi could be colonized in sterilized soil and effectively degraded DCD.Application of urea＋ DCD ＋exogenous fungi,the population of soil fungi increased of 36.40×105 cfu and decreased significantly DCD content after 15 d of incubation.The degradation of DCD was shown close relationship with soil microorganism.Exogenous fungi enhanced the degradation of DCD in soils.

关 键 词：双氰胺 尿素 碳酸氢铵 降解菌 降解 

分 类 号：X53[环境科学与工程—环境工程]