SPE-GC法同时检测草莓中7种常用农药的残留  

Simultaneous Determination of the Residue of Seven Commonly-used Pesticides in Strawberry with Solid phase Extraction and Gas Chromatography

作　　者：杨振华[1] 魏朝俊[1] 贾临芳[1] 梁丹[1] 赵建庄[1] 

机构地区：[1]北京农学院,北京102206

出　　处：《农业环境科学学报》2012年第11期2304-2308,共5页Journal of Agro-Environment Science

基　　金：2011第七届北京市教学名师项目;北京市属高等学校人才强教深化计划-学术创新人才(PHR201006124);北京市属高等学校人才强教深化计划(PHR201108278);北京市属高等学校人才强教深化计划(PHR200907136);农产品加工及贮藏工程北京市重点建设学科(PXM2009-014207-078172);首都农产品安全产业技术研究院成果转化项目(PXM2012-014207-000019);北京市教委科技面上项目(KM201210020014);北京市优秀人才培养项目(2010D005021000005)

摘　　要：为检测草莓基质中的农药残留,建立了固相萃取-毛细管柱气相色谱方法,可同时检测草莓果实中百菌清、毒死蜱、粉唑醇、腈菌唑、高效氯氰菊酯、氰戊菊酯和嘧菌酯7种农药的残留量。样品经乙腈提取,C18固相萃取柱净化,GC-ECD进行定性及定量分析。百菌清、毒死蜱在0.05～1.0μg.mL-1,粉唑醇、腈菌唑、高效氯氰菊酯、氰戊菊酯和嘧菌酯在0.5～10.0μg.mL-1浓度范围内呈现良好线性关系。方法平均回收率为85.3%～102.7%,RSD小于5%,检出限范围为0.005～0.132mg.kg-1。该方法简单、快速、灵敏、准确,能满足国标中相关农药残留限量测定的要求。A new method was developed to simultaneously detect the residue of 7 pesticides（chlorothalonil,chorpyriphos,flutriafol,myclobutanil,beta-cypermethrin,fenvalerate and azoxystrobin）in strawberry with solid-phase extraction and gas chromatography.The strawberry samples were extracted with acetonitrile,purified with C18 solid-phase extraction column,and detected by GC-ECD.The calibration curves were of good linearity when chlorothalonil and chorpyriphos were within a range from 0.05 μg.mL-1 to 1.0 μg.mL-1,and flutriafol,myclobutanil,beta-cypermethrin,fenvalerate and azoxystrobin were from 0.5 μg.mL-1 to 10.0 μg.mL-1.The average recovery rates ranged from 85.3% to 102.7%,its relative standard deviation（RSD）were lower than 5%,and the limit of detection（LOD）for all compounds changed from 0.005 mg.kg-1 to 0.132 mg.kg-1.This method is simple,rapid,delicate,accurate,and can meet the relevant requirements of determining pesticide residues in GB.

关 键 词：草莓 气相色谱法 农药残留 检测 

分 类 号：X836[环境科学与工程—环境工程]