X射线全身照射对大鼠血清氨基转移酶活性和肝脏结构的影响  

Effects of Whole Body X-Ray Irradiation on Serum Transaminase Activity and Liver Structure in Rats

作　　者：曲保忠[1] 王新成[2] 孙丹丹[1] 徐俊杰[3] 吕士杰 

机构地区：[1]吉林医药学院口腔影像系,吉林吉林132013 [2]吉林医药学院直属医院,吉林吉林132013 [3]吉林医药学院生化教研室,吉林吉林132013

出　　处：《环境与职业医学》2012年第11期686-688,共3页Journal of Environmental and Occupational Medicine

基　　金：吉林省教育厅十一五科学技术研究重点资助项目(编号:吉教科合字[2010]第250号)

摘　　要：[目的]观察不同剂量X射线全身照射对大鼠血清氨基转移酶活性及肝脏结构的影响。[方法]Wistar大鼠40只,随机分为4组:对照组、2Gy组、4Gy组、6Gy组。除对照组外,其余3组大鼠按处理剂量行X射线全身单次照射。照射后第2天,经腹主动脉取血、分离血清,测量谷氨酸氨基转移酶(alanine transaminase,ALT)、天冬氨酸氨基转移酶(aspartate transaminase,AST);取肝脏组织制作病理切片,观察肝脏的光学显微镜、电子显微镜结构。[结果]4Gy、6Gy组ALT水平升高,与对照组相比,差异具有统计学意义(P<0.01);各照射组AST值与对照组相比,差异无统计学意义(P>0.05)。光镜结果显示,各照射组未见明显异常;电镜结果显示,6Gy组细胞器的改变多见于内质网,其结构松散,出现大量空泡。[结论]4Gy、6Gy X射线全身照射可对大鼠血清氨基转移酶活性造成影响,6Gy X射线照射可对肝脏结构造成损害。[ Objective ] To study the effects of whole body X-ray irradiation on serum transaminase indices and structure of liver in rats. [ Methods ] Forty Wistar rats were randomly divided into four groups as blank control and other groups exposed to varied doses of X-ray （2 Gy, 4 Gy, and 6 Gy）. Except those in the control group, the other 3 groups of rats were exposed to a single X-ray irradiation to the whole body with the designed doses. On the second day after irradiation, the serum alanine transaminase （ALT） and aspartate transaminase （AST） were assayed by sampling blood from abdominal aorta. The structure of hver was observed under optical microscope and electron microscope, [ Results ] Compared with the control group, the ALT levels of the 4Gy and 6 Gy groups were significantly elevated （P 〈 0.01）. There was no significant differences in the AST levels between the control group and the irradiation groups （P 〉 0.05）. No apparent changes in hepatic structure were found under the optical microscope. However, vacuolated endocytoplasmic reticulum was identified in the 6 Gy group under electron microscope. [ Conclusion ] The serum transaminase indices of rats are changed after X-rays irradiation at 4 Gy and 6 Gy, and 6 Gy irradiation could damage hepatic structure.

关 键 词：X射线 血清氨基转移酶 超微结构 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]