超高效液相色谱法同时测定知母中芒果苷等6种成分的含量  

UPLC Determination of Six Active Components Including Mangiferin in Anemarrhenae Rhizoma

作　　者：罗洁[1] 石绍淮[1] 张靓琦[1] 范旭航[1] 毕开顺[2] 贾英[1] 

机构地区：[1]沈阳药科大学中药学院,沈阳110016 [2]沈阳药科大学药学院,沈阳110016

出　　处：《中国药学杂志》2012年第22期1856-1859,共4页Chinese Pharmaceutical Journal

基　　金：辽宁省科学技术计划项目资助(2011412004);辽宁省教育厅创新团队项目资助(2009T097)

摘　　要：目的建立同时测定知母中新芒果苷,芒果苷,知母皂苷BⅡ,2,6,4'-三羟基-4-甲氧基苯甲酮,构树宁B,顺-扁柏树脂酚的UPLC方法。方法采用ACQUITY UPLC,HSS T3色谱柱(2.1 mm×100 mm,1.8μm),流动相为乙腈-0.03%的磷酸水溶液梯度洗脱,流速为0.5 mL.min-1,检测波长采用210和192 nm。结果新芒果苷,芒果苷,知母皂苷BⅡ,2,6,4'-三羟基-4-甲氧基苯甲酮,构树宁B,顺-扁柏树脂酚的质量浓度与峰面积分别在15.00～299.6μg.mL-1,8.80～175.6μg.mL-1,40.00～800.0μg.mL-1,2.300～45.60μg.mL-1,0.420～8.50μg.mL-1,0.400～9.00μg.mL-1内呈良好的线性关系。平均加样回收率(n=9)均在97.7%～100.6%之间,RSD均小于1.2%。结论该方法快速、准确、简便,可作为同时测定知母中新芒果苷,芒果苷,知母皂苷BⅡ,2,6,4'-三羟基-4-甲氧基苯甲酮,构树宁B,顺-扁柏树脂酚的方法。OBJECTIVE To establish an analytical method to determine 7-O-glucopyranosylmangiferin, mangiferin, timosapon- inB Ⅱ , 2,6,4＇-trihydroxy-4-methoxybenzophenone, broussonin B and cis-hinokiresinol in Anemarrhenae Rhizoma quickly and accurate- ly. METHODS A UPLC method was established on an HSS T3 column （2.1 mm ×100 mm, 1.8 μm）. The mobile phase consisted of 0.03% phosphoric acid solution and acetonitrile eluted in gradient mode, and the detection wavelength was set at 192 and 210 nm. RESULTS The standard curves of 7-O-glucopyranosylmangiferin, mangiferin, timosaponin B Ⅱ , 2, 6, 4＇-trihydroxy-4-methoxy- benzophenone, broussonin B and c/s-hinokiresinol showed good linearity in 15.00 -299.6 μg · mL-l, 8.80 - 175.6 μg · mL-1, 40. 00 - 800. 0 μg · mL -1, 2. 300 - 45.60 μg · mL -1, 4. 200 - 8.50 μg · mL -1 and 0. 400 - 9. 00 μg · mL -1 , respectively. The mean recoveries （ n = 9） of the 6 components were 97.7% - 100. 6% with RSD of 1.2%. CONCLUSION The method is quick and accurate and can be used to determine 7-O-glucopyranosylmangiferin, mangiferin, timosaponin B Ⅱ, 2,6,4＇-trihydroxy-4-methoxy- benzophenone, broussonin B and cis-hinokiresinol in Anemarrhenae Rhizoma.

关 键 词：知母 超高效液相色谱法 新芒果苷 芒果苷 知母皂苷BⅡ 

分 类 号：R917[医药卫生—药物分析学]