亚低温对短暂性脑缺血再灌注老龄大鼠海马葡萄糖调节蛋白78表达的影响  

Effect of mild hypothermia on glucose regulated protein 78 expression in hippocampus following transient cerebral ischemia-reperfusion in aged rats

作　　者：刘孝洁[1] 时飞[1] 毕燕琳[1] 陈怀龙[1] 李颖[1] 王明山[1] 

机构地区：[1]青岛大学医学院附属青岛市市立医院麻醉科,266071

出　　处：《中华麻醉学杂志》2012年第9期1130-1132,共3页Chinese Journal of Anesthesiology

摘　　要：目的探讨亚低温对短暂性脑缺血再灌注老龄大鼠海马葡萄糖调节蛋白78（GRP78）表达的影响。方法健康雄性老龄SD大鼠144只，体重450～550g，采用随机数字表法，将其随机分为3组（n=48）：假手术组（S组）、脑缺血再灌注组（I／R组）、全身亚低温组（H组）。采用四血管阻断法建立全脑缺血再灌注模型，脑缺血5min再灌注。H组于再灌注即刻向大鼠全身喷洒酒精行体表降温，维持直肠温32～34屯3h。分别于再灌注6、12、24,48h时，采用免疫组织化学方法和Westernblot法测定海马GRP78的表达；HE染色法计数海马存活神经元数目。结果与s组相比，I／R组再灌注各时点海马存活神经元计数降低，再灌注6、12、24h时海马GRP78表达上调，再灌注48h时表达下调，H组再灌注各时点海马存活神经元计数降低，海马GRP78表达上调（P〈0．05）；与I／R组相比，H组再灌注12、24和48h时海马存活神经元计数升高，再灌注各时点GRP78表达上调（P〈0．05）。结论亚低温可进一步上调老龄大鼠短暂性脑缺血再灌注时海马GRP78的表达，从而减轻内质网应激反应，减轻脑损伤。Objective To investigate the effect of mild hypothermia on glucose regulated protein 78 （GRP78） expression in hippocampus following transient cerebral ischemia-reperfusion （I/R） in aged rats. Methods One hundred and forty-four male aged Sprague-Dawley rats, weighing 450-550 g, were randomly divided into 3 groups （ n = 48 each） ： sham operation group （group S） , I/R group and mild hypothermia group （group H） . Glo- bal cerebral I/R was induced by 4-vessel occlusion method. Bilateral vertebral arteries were permanently occluded by cauterization, and bilateral carotid arteries were occluded for 5 min. The surface cooling was started immediately after reperfusion and maintained for 3 h. During surface cooling, the body temperature was maintained at 32-34 ℃ （rectal） . At 6, 12, 24 and 48 h of reperfusion, the expression of GRP78 was determined using immuno-histo- chemisty and Western blot, and the viable neurons in CAI area were counted. Results The number of viable neu- rons was significantly smaller at each time point, and the expression of GRP78 was significantly higher at 6, 12 and 24 h of reperfusion, while lower at 48 h of reperfusion in I/R group, and the number of viable neurons was signifi- cantly smaller, and the expression of GRP78 was significantly higher at each time point in group H than in group S （ P 〈 0.05） . The number of viable neurons was significantly larger at 12, 24 and 48 h of reperfusion, and the ex- pression of GRP78 was significantly higher at each time point in group H than in group I/R （ P 〈 0.05）. Conclu- sion Mild hypothermia can further up-regulate GRP78 expression in hippocampus following transient cerebral I/R in aged rats, thus reducing endoplasmie reticulum stress and cerebral I/R injury.

关 键 词：低温 人工 热休克蛋白类 再灌注损伤 脑 老年人 

分 类 号：R285.5[医药卫生—中药学]