治咳川贝枇杷滴丸UPLC-Q-TOF-MS^E指纹图谱研究  

UPLC-Q-TOF-MS^E fingerprint investigations of Zhikechuanbeipipa dropping pills

作　　者：罗艺[1] 白钢[2] 罗国安[2] 齐欣[3] 张宁[1] 郭华[1] 董林毅[4] 

机构地区：[1]天津医科大学肿瘤医院肿瘤研究所,天津300601 [2]南开大学药学院,天津300071 [3]天津中新药业集团股份有限公司第六中药厂,天津300401 [4]天津医科大学药学院,天津300070

出　　处：《药物分析杂志》2012年第11期1940-1944,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：973计划项目(2011CB933104);国家自然基金面上项目(No.81173638);国家自然基金青年专项(No.81102835)

摘　　要：目的:全面、快速阐明复方中药治咳川贝枇杷滴丸的化学组成。方法:采用UPLC-Q-TOF-MSE对治咳川贝枇杷滴丸的化学成分进行快速表征、鉴定,并尝试使用其特征指纹图谱进行质量控制。结果:对治咳川贝枇杷滴丸进行超声波辅助结合低温冻提,采用UPLC-Q-TOF-MSE对其醇提物中多种化合物进行分析,从中鉴定了35个化合物。在此基础上,以紫外谱图的24个共有峰建立了治咳川贝枇杷滴丸的指纹图谱,并对不同批次滴丸进行了相似度分析,表明不同批次治咳川贝枇杷滴丸的质量差异较小。结论:本方法对治咳川贝枇杷滴丸化学成分的快速鉴定及质量控制奠定了基础。Objective： To develop a comprehensive and rapid method for elucidation of the chemical composition and determination of the characteristic chromatographic profiles of a traditional prescription Zhikechuanbeipipa dropping pills, and hence to control its quality. Methods： An UPLC -Q -TOF- MSE method was developed. Thirty- five components were detected from the prescription after ultrasonic extraction at -20 ~C. Results： A Zhikechuanbeipipa dropping pills fingerprint was built based on 24 common peaks in the PDA chromatographic pro- file. The 24 common peaks were selected according to the similarity of ten batches Zhikeehuanbeipipa dropping pills. Conclusion： The characteristic chromatographic profile of Zhikechuanbeipipa dropping pills with high speci- ficity can be used for the quality control of the prescription.

关 键 词：治咳川贝枇杷滴丸 超高效液相色谱 高分辨串联质谱 指纹图谱 快速表征 相似度分析 

分 类 号：R917[医药卫生—药物分析学]