黄连4种饮片特征图谱研究及生物碱含量测定  

HPLC specific chromatogram study and determination of alkaloids from four kinds of Huanglian(Coptis chinensis Franch.) decoction pieces

作　　者：张永鑫[1] 刘晓[1] 杨欣文[1] 彭稳稳[1] 李俊松[1] 蔡宝昌[1] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室,南京210029

出　　处：《药物分析杂志》2012年第11期1957-1961,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：江苏省高校自然科学研究重大项目(10KJA360038)

摘　　要：目的:建立黄连4种不同炮制品的特征图谱,探讨饮片炮制前后化学成分的变化规律,并采用HPLC法对其中木兰碱、药根碱、黄连碱、巴马汀、小檗碱5个生物碱类成分进行定量分析比较。方法:采用Kromasil-C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-50 mmol.L-1磷酸二氢钾溶液(磷酸调pH 3.0)(45∶55),内含十二烷基硫酸钠12.5 mmol.L-1;检测波长275 nm;流速1 mL.min-1;柱温为30℃;进样量10μL。结果:所建立的特征图谱信息丰富,5个生物碱成分在测定范围内线性关系较好(r>0.9994),平均回收率分别为102.8%,96.9%,98.4%,104.8%,103.7%。结论:初步实验表明,黄连不同炮制品的特征图谱有所差别,相关成分在炮制前后发生了量变和质变,并呈现一定的变化规律。Objective： To develop the HPLC specific chromatograms of four kinds of processed Rhizoma Coptis, to explore the regularity of component changes in processing of Rhizoma Coptis, and use HPLC to quantitatively ana- lyze and compare magnoflorine, jatrorrhizine, coptisine, palmatine, berberine proportions . Method： The separa- tion was performed on an Kromasil -Cl8 column（250 mm× 4.6 mm, 5 μm）with the mobile phase consisting of acetonitrile - 0. 05 mol L - 1 potassium phosphate （ adjusting pH = 3.0 with phosphoric acid ） （45： 55 ）, containing 12.5 mmol L-1 SDS; The column temperature was set at 30 ℃. The flow rate was 1.0 mL min-1 and the injec- tion volume was 10 μL. Detection wavelength was set at 275 nm. Results： The information of HPLC specific chro- matogram was abundant. Other components＇ proportion was increasing in different level except coptisine and epiberberine in Rhizoma Coptis processed with wine ; the majority of components＇ proportion increased obviously in Rhizoma Coptis processed with ginger juice ; other components＇ proportion increased slightly except magnoflorine in Rhizoma Coptis processed with Fructus Evodiae ; The linear correlation of five alkaloids are good （r 〉 0. 9994 ） , The average recoveries of five components were 102. 8% ,96. 9% ,98.4%, 104. 8%, and 103.7%. Conclusion： The HPLC specific ehromatograms were varied in different processed products, all the component changed in quantity and quality after processing, and some regularity of changing was observed

关 键 词：黄连炮制品 生物碱 特征图谱 成分变化规律 高效液相色谱法 含量测定 木兰碱 药根碱 黄连碱 巴马汀 小檗碱 

分 类 号：R917[医药卫生—药物分析学]