H9N2亚型禽流感病毒细胞灭活疫苗的免疫效果评价  

Immunity Evaluation on Inactivated Cellular Vaccine of H9N2 Subtype Avian Influenza Virus

作　　者：刘建龙[1,2] 滕巧泱[2] 范钊[2] 胡涛[2] 李雪松[2] 李国新[2] 葛铭 李泽君[2,3] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]中国农业科学院上海兽医研究所,上海200241 [3]中国农业科学院上海兽医研究所农业部寄生虫重点开放实验室,上海200241

出　　处：《中国家禽》2012年第21期24-27,共4页China Poultry

基　　金：公益性行业(农业)科研专项(201003012);国际交流与合作特别基金(S2011ZR0429);国家高技术研究发展计划(2011AA10A200)

摘　　要：由于经济和技术因素的制约,尚未有成功的禽流感细胞疫苗产品上市。为此,本研究在细胞扩增而来的含有H9亚型禽流感病毒(AIV)HA和NA基因的SH441HANAPR8(NS mutant)重组病毒里加入终浓度为0.1%的甲醛进行灭活。结果显示,37℃条件下灭活24h的效果最佳。灭活好的H9AIV SH441HANAPR8(NS mutant)与佐剂MontanideISA70VG乳化制备成灭活苗,以0.05、0.1、0.2、0.3mL/只的剂量,分别胸部肌肉注射免疫3周龄的SPF鸡,并于免疫后21d静脉感染A/Chicken/Shanghai/441/2009(H9N2)AIV。结果显示,该疫苗的最小免疫剂量为0.2mL。此外,研究还发现,当HI抗体效价≥25时,疫苗对鸡只的攻毒保护率达到100%。Restricted by the economic and technological factors, avian influenza cellular vaccine has not been in market. Therefore,reassotant virus SH441HANAPR8 （NS mutant）that contained HA and NA genes from H9 subtype avian influenza virus acquired by cell culture was used in this paper. It was the best to mix SH441HANAPR8 （NS mutant）with formaldehyde to a final concentration of 0.1% and inactivated at 24 h. The inactivated SH441HANAPR8 （NS mutant） viruses were mixted with adjuvant Montanide ISA 70VG,and emulsified to inactivated vaccine. By chest muscles pathway, 3-week-age specific pathogen free （SPF） chickens were immunized with H9 avian influenza virus inactivated vaccine （SH441HANAPR8 （NS mutant）in different doses of 0.05,0.1,0.2 and 0.3 mL per chicken. After 21 days post-immunization, these chickens were infected with avian influenza virus A/Chicken/Shanghai/441/2009 （H9N2）. The results demonstrated that minimum immune dose of the vaccine was 0.2 mL per chicken. In addition,when HI titer was equal or greater than 2^5,challenge protection rate of vaccine was 100% for chicken.

关 键 词：H9N2亚型禽流感病毒 最小免疫剂量 免疫 

分 类 号：S852.659.5[农业科学—基础兽医学]