家禽活疫苗中鸡传染性贫血病毒PCR检测方法的建立  

A Rapid PCR Approach for Detecting the Contamination of Chicken Anemia Virus in Avian Live Vaccines

作　　者：邵红霞[1] 王平平[1] 武敏[1] 金文杰[1] 钱琨[1] 秦爱建[1] 

机构地区：[1]扬州大学兽医学院,禽类预防医学教育部重点实验室,江苏省动物预防医学重点实验室,江苏扬州225009

出　　处：《中国家禽》2012年第21期28-30,共3页China Poultry

基　　金：教育部创新团队项目(IRT0978);江苏高校优势学科建设工程资助项目

摘　　要：为建立不依赖于病毒分离的快速,特异的禽活疫苗中鸡传染性贫血病毒(CAV)监测方法,本研究建立了CAV的PCR检测方法。试验表明:该PCR不仅具有良好的CAV特异性,且检测灵敏度达3.98个TCID50/反应。用该方法监测9份随机抽取的商品禽用活疫苗发现,其中2份活疫苗呈PCR强阳性。以上结果表明,本研究建立的鸡传染性贫血病毒PCR检测方法具有特异、敏感等特点,能用于禽用活疫苗中CAV污染的快速监测。To monitor chicken anemia virus （CAV） in avian live vaccines without isolating viruses, a rapid PCR method for detection of CAV was developed. Results showed that PCR had high CAV specificity and the minimum detection was 3.98 TCID50/reaction. The PCR application showed two of commercial avian live vaccines were CAV positive. Over all,the PCR approach developed here not only showed high specificity and sensitivity for CAV detection, but also had great potential to be applied in detecting CAV in avian live vaccines.

关 键 词：鸡传染性贫血病毒 PCR 疫苗 监测 

分 类 号：S852.65[农业科学—基础兽医学]