小麦谷氨酰胺合成酶基因克隆与其表达特性分析  

Cloning of glutamine synthetases in wheat and analysis of their expression characteristics

作　　者：王小纯[1] 张同勋[1] 李高飞[1] 程振云[1] 刘宁[1] 马新明 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002 [2]河南省粮食作物生理生态与遗传改良重点实验室,河南郑州450002

出　　处：《河南农业大学学报》2012年第5期487-492,共6页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(30771266);河南省高校创新人才基金项目(2010HASTIT004)

摘　　要：为了研究小麦谷氨酰胺合成酶(Glutamine synthetase,GS)的功能,利用RACE及RT-PCR技术克隆了小麦GS1(GenBank登录号:HQ840647)和GS2(GenBank登录号:JF894116)基因的全长cDNA.小麦GS1的cDNA全长为1 494 bp,GS2的cDNA全长为1 631 bp,并利用生物信息软件对GS1和GS2的基因序列以及所表达的蛋白特性进行了分析.利用半定量PCR和Western-bolt技术分别分析了小麦苗期叶片发育过程中GS同工酶基因转录和表达特点,结果表明,GS1在叶片开始衰老时转录和表达水平较高,GS2从叶片伸长期到定长期转录和表达水平最高.In order to study the function of glutamine synthetase （GS） in wheat, the full-length cDNA of GS1 and GS2 were cloned by RACE and RT-PCR （The accession number of GS1 is HQ840647, and GS2 is JF894116）. The full length cDNA of GS1 is 1 494 bp and that of GS2 is 1 631 bp. The se-quences and protein characteristics of GS1 and GS2 were analyzed with the biological software. Semi-quantitative PCR and western-blot were used to demonstrate the transcriptional and expressional levels of GS genes in wheat leaf during seedling stage, and the results showed that both levels of GS1 got high-er when the leaf began to senescence while both levels of GS2 kept the highest from the leaf expanding stage to the full expansion stage.

关 键 词：小麦 谷氨酰胺合成酶 基因克隆 基因转录 基因表达 

分 类 号：S512.1[农业科学—作物学]