家蚕性信息素腺体肌质网膜Ca^(2+)-ATP酶基因的分子鉴定  

Molecular identification of sarco/endoplasmic reticulum Ca^(2+)-ATPase gene involved in PBAN-stimulated pheromone production in Bombyx mori

作　　者：张松斗[1] 陈丽君[1] 安世恒[1] 杜孟芳[1] 

机构地区：[1]河南农业大学植物保护学院,河南郑州450002

出　　处：《河南农业大学学报》2012年第5期577-583,共7页Journal of Henan Agricultural University

基　　金：河南省自然科学基金项目(2011B180021)

摘　　要：对肌质/内质网膜Ca2+-ATPase(Sarco/endoplasmic reticulum Ca2+-ATPase,SERCA)基因在家蚕雌虫性信息素腺体内的分子特性进行了研究.结果表明,羽化当天该基因在性信息素腺体内的表达量最高.组织表达模式发现,该基因在性信息素腺体、头、体壁、脂肪体、飞行肌、卵等组织中均有表达,但在卵中的表达量相对较低,在中肠中不表达.断头后SERCA基因的表达仍然呈现上升趋势,说明该基因的表达是时间依赖型的,但与正常发育的雌虫相比,断头明显抑制了SERCA基因的表达,保幼激素也明显抑制了该基因的表达.In the present study, the molecular characteristics of sarco/endoplasmic reticulum Ca^2＋ - ATPase （SERCA） gene from pheromone glands were studied. Temporal expression analysis revealed that Bombyx mori SERCA （BmSERCA） showed the highest expression in new-emerged adults. The spatial expression analysis suggested that BmSERCA was expressed in pheromone gland, head, cuticle, fat body, muscle, egg. However, it was weakly expressed at eggs and there was no expression in mid-gut. BmSERCA still continued to increase despite the decapition treatment, suggesting that the ex-pressed pattern of BmSERCA was age-dependent. Compared with normal female, decapitation signifi-cantly inhibited the expression of BmSERCA. Juvenile hormone treatments significantly inhibited the expression of BmSERCA.

关 键 词：家蚕 PBAN 性信息素 CA2+-ATP酶 保幼激素 

分 类 号：Q966[生物学—昆虫学]