糖芯片制备和应用的最新研究进展  

Modern Carbohydrate Microarray Bio-chip Technologies

作　　者：万锕俊[1] 王琨[2] 张洪才[1,2] 李慧丽[3] 王德农 

机构地区：[1]上海交通大学化学与化工学院,上海200240 [2]上海交通大学农业与生物学院,上海200240 [3]上海交通大学药学院,上海200240 [4]Tumor Glycomics Laboratory,Center for Cancer Research,SRI International Biosciences Division,CA94025-3493,USA

出　　处：《分析化学》2012年第11期1780-1788,共9页Chinese Journal of Analytical Chemistry

基　　金：中国国家自然科学基金项目(No 51173104);上海纳米专项(No.11nm0503500);美国NIH基金项目(No.CA128416)资助

摘　　要：糖芯片是一种研究微量糖与生物大分子之间相互作用的生物检测技术,因其具有用量少、快速、高效和高通量等特点,现已被广泛应用到药物开发、免疫学,临床诊断和细菌检测等诸多领域中。近年来,尽管对糖芯片的制备方法和应用进行了较为深入的研究,但对糖芯片的制备方法和应用的综述还较少报道。本文主要介绍糖芯片的制备原理、非化学修饰和化学修饰制备糖芯片的最新方法,然后对糖芯片在自组装等方面应用的最新进展进行综述,并对糖芯片所遇到的挑战和发展趋势也作了展望。Carbohydrate microarray（glyco-chip）,is one of modern biotechnologies for monitoring the molecular interactions between carbohydrate ligands and other biomacromolecules.It is characterized by micro-scale,quickness,high sensitivity and high-throughput and is widely applicable in many fields.Its promising RD areas include,but are not limited to,biology,basic medical research, clinical diagnostics,drug development and bio-material reagent industry.This article intends to summarize methods that are currently used for the construction of glyco-chips,such as immobilization of either chemically modified or non-modified carbohydrates and procedures of self-assembly for production of three-dimensional glyco-chips.Current challenges and future trends in the RD of glyco-chips are also discussed.

关 键 词：微阵列 生物芯片 糖芯片 自组装 综述 

分 类 号：Q819[生物学—生物工程]