双重RT-PCR法同时快速检测南方水稻黑条矮缩病毒和水稻黑条矮缩病毒  

Quick Detection of SRBSDV and RBSDV by Duplex RT-PCR

作　　者：孙晓棠[1] 崔汝强[1] 贺浩华[1] 欧阳林娟[1] 胡丽芳[1] 彭小松[1] 陈小荣[1] 朱昌兰[1] 

机构地区：[1]江西农业大学农学院/作物生理生态与遗传育种教育部重点实验室,江西南昌330045

出　　处：《江西农业大学学报》2012年第5期914-917,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家"十二五"科技支撑计划项目(2012BAD14B1);高等学校博士学科点专项科研基金(20113603110001);转基因生物新品种培育重大专项(2011ZX08001-002);江西省重大科技专项(20114ABF03102)

摘　　要：南方水稻黑条矮缩病毒(SRBSDV)和水稻黑条矮缩病毒(RBSDV)在病害症状、传播介体及寄主等方面非常相似,田间很难对其进行诊断及鉴定。根据SRBSDV与RBSDV外壳蛋白(CP)基因核苷酸序列差异设计特异性引物,建立一种快速、准确的双重PT-PCR鉴别方法,为SRBSDV和RBSDV的流行监测提供技术支持。Southem rice black-streaked dwarf virus （SRBSDV） and rice black-streaked dwarf virus （RBSDV） are two important virus diseases in rice. They share many similar characters, such as symptoms, vectors and host range etc. , causing difficulty in discrimination of the two viruses. In this study, three primers were designed based on the reported CP gene, and a duplex RT-PCR protocol for the detection of two viruses was developed, which provides a rapid and specific method for simultaneous detection of SRBSDV and RBSDV.

关 键 词：南方水稻黑条矮缩 水稻黑条矮缩病毒 外壳蛋白基因 特异性检测 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]