辣椒小G蛋白CaRab11基因全长cDNA的分离及序列分析  

Isolation and Sequence Analysis of a Full-length cDNA Clone Encoding Small GTP-Binding Protein Gene CaRab11 in Pepper

作　　者：赖燕[1] 林金辉[1] 陈成聪[1] 官德义[2] 牟少亮[2] 邱爱连[1] 何水林[1,2] 

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建农林大学作物科学学院,福建福州350002

出　　处：《江西农业大学学报》2012年第5期1021-1025,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30600391;30971718);高校博士点基金(20093515110004);福建省自然科学基金项目(2008J0049)

摘　　要：通过分析克隆获得的辣椒Rab11全长cDNA及其编码的氨基酸序列结构特征,为进一步研究辣椒Rab11功能奠定基础。通过对辣椒均一化cDNA文库的筛选分离获得了一个与葡萄Rab11小G蛋白VvRabA1f高度同源的全长cDNA,命名为CaRab11。序列分析结果表明:该cDNA包含有1 164 bp的完整开放阅读框,编码217个氨基酸。该蛋白含有Rab GTP酶超家族保守的RabSF模体;Rab亚家族蛋白所特有的五个氨基酸序列RabF模体(RabF1-RabF5);参与GTP/Mg++结合及GTP水解的G结构域(G1-G5:GDSGVGKS,T,DTAGQE,GNKADL及ETSAL);两个构象变构域(SwitchⅠ和SwitchⅡ)及与异戊二烯化相关的CCX序列。氨基酸同源性及进化分析同样表明CaRab11为辣椒小G蛋白Rab11家族新成员。The characteristics of Rabll cDNA and deduced amino acids sequence in pepper were ana- lyzed, which would lay the foundation for further investigation of the function of Rabll protein. One Ⅰ 164 bp fulllength cDNA clone was isolated from a pepper normalized cDNA library, which encodes a putative protein composed of 217 amino acids. The full-length cDNA was named CaRabl I. The amino acid sequence deduced by CaRabll cDNA showed high similarity to VvRabAlf protein from Vitis vinifera. CaRabll protein included conserved domains specific to Rab GTPase superfamily （RabSF）, Rab subfamily specific regions （RabF, RabF1 -RabF5 ）, five highly conserved GTP- binding consensus sequences （GDSGVGKS, T, DTAGQE, GNKADL, and ETSAL） involved in GTP! Mg ＋ ＋ binding and GDP hydrolysis, two conformation- al switch （ Switch I and II） regions as well as Cterminal cysteines motif important to prenylation. Amino acids similarity and phylogenetic analysis also indicated that CaRab11 is a new member of small GTP - binding protein Rabll superfamily.

关 键 词：辣椒 小G蛋白 基因克隆 序列分析 

分 类 号：S641.3[农业科学—蔬菜学] Q78[农业科学—园艺学]