海南地区剑叶龙血树种质资源遗传多样性分析  

Genetic diversity of Dracaena cochinchinensis germplasm resources in Hainan province

作　　者：杨松[1] 莫饶[1] 唐燕琼[1] 朱文丽 黄明忠 李永杰[1] 谢添伟[1] 

机构地区：[1]海南大学农学院,海南儋州571737 [2]中国热带农业科学研究院热带作物品种资源研究所,海南儋州571737

出　　处：《广东农业科学》2012年第19期124-125,133,共3页Guangdong Agricultural Sciences

基　　金："211工程"热带作物遗传育种与生态保育创新人才培养基金(YJS-2011-0203)

摘　　要：利用ISSR标记法对海南省野生剑叶龙血树资源进行多样性分析，从100条ISSR引物中选取10条多态性好、重复性高、谱带清晰的引物，对105份样品DNA进行扩增，共得到116条谱带，其中多样性谱带有104条。品种间遗传相似系数在O．6442～0．994之间，平均为0．8011，表明剑叶龙血树遗传基础相对较狭窄。利用UPGMA聚类分析，将7个样地的居群分为1个大类群和1个小类群，类群与地理分布密切相关。Diversity of Hainan wild Dracaena cochinchinensis resourse was analyzed by ISSR markers, 10 primer pairs were selected from 100 ISSR primers as a result of better polymorphism, high repeatability, clear strips. A total of 116 bands was obtained, including 104 polymorphic bands. The range of GS was 0.6442-0.994, averaged 0.8011, which indicated a narrow genetic basis of D. cochinchinensis. Groups from 7 sample plot were divided into a large group and a small group, clustered by UPGMA method. Close relationship was found between the division of groups and local sources.

关 键 词：剑叶龙血树 ISSR 遗传多样性 

分 类 号：Q346.5[生物学—遗传学]