Rip1-Tag2;P-sel^(-/-)小鼠模型的构建  

The Construction of Rip1-Tag2;P-sel^(-/-) mice Model

作　　者：亓翠玲[1] 叶杰[1] 郭四美[1] 周秦[1] 韩露[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物学研究所,广州510006

出　　处：《中国医疗前沿》2012年第19期8-9,共2页China Healthcare Innovation

基　　金：国家自然科学基金资助项目(编号:30900764);广东省医学科研课题(编号:A2011299)

摘　　要：目的构建Rip1-Tag2;P-sel-/-小鼠模型。方法将Rip1-Tag2小鼠与P-选择素敲除(PKO)小鼠杂交,剪尾提DNA后用PCR法检测小鼠的基因型。结果 Rip1-Tag2小鼠与PKO小鼠杂交三代后,Rip1-Tag2;P-sel-/-小鼠模型构建成功。结论将Rip1-Tag2小鼠与PKO小鼠杂交三代后成功建立了Rip1-Tag2;P-sel-/-小鼠模型,并进行繁育。Objective To construct the Ripl-Tag2;P-sel-/- mice. Methods Female PKO mice were mated with male Ripl-Tag2 mice and male progeny carrying the Ripl-Tag2 transgene. Mice were genotyped by PCR. Result After female PKO mice being mated with male Ripl-Tag2 mice for three generations, Ripl-Tag2;P-sel /- mice were obtained. Conclusion Ripl-Tag2; P-sel-/- mice were obtained and propagated through Ripl-Tag2 mice being mated with PKO mice.

关 键 词：P-选择素 Rip1-Tag2转基因小鼠 P-选择素敲除小鼠 动物模型 胰腺肿瘤 

分 类 号：R392-33[医药卫生—免疫学]