HPLC测定野马追中野马追内酯A,B和金丝桃苷的含量  

Determination of Eupalinolide A,B and Hyperin in Eupatorium Lindleyanum DC by HPLC

作　　者：罗敏敏[1] 李春华[1] 王易芬[2] 

机构地区：[1]中南大学化学化工学院,长沙410083 [2]中国科学院昆明植物所,昆明650201

出　　处：《中国现代应用药学》2012年第11期1018-1022,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的采用高效液相色谱法对不同产地的6批野马追药材中野马追内酯A，B和金丝桃苷进行了定性定量分析。方法野马追内酯A，B的测定采用AgilentExtend．C18（4．6mm×150mm，5μm）为分析柱，乙腈-水（27：73）为流动相，流速为1．0mL·min-1，等度洗脱，检测波长为220nm，柱温为30℃。金丝桃苷的测定以SB—C18为分析柱，乙腈-1％醋酸水溶液（12：88）为流动相，流速为1．0mL·min-1，等度洗脱，检测波长为255nm。结果野马追内酯A在0．02-1．88μg，野马追内酯B在0．44～4．44μg内与峰面积呈良好的线性关系。金丝桃苷在0．101～1．414μg内与峰面积呈良好的线性关系。结论本实验方法准确、快速、灵敏、重复性好，在该色谱条件及样品处理方法下，可以准确测定野马追中野马追内酯A，B和金丝桃苷的含量。OBJECTIVE To establish a method for the determination of the content of eupalinolide A, B and hyperin in Eupatorium lindleyanum DC from 6 different areas by HPLC. METHODS For eupalinolide A, B, Agilent Extend-Cl8 column （4.6 mm×150 mm, 5μm） was used under the mobile phase of acetonitrile-water（27 ： 73） at the flow rate of 1.0 mL min-1. The detection wavelength was 220 nm and the column temperature was maintained at 30 ℃. For hyperin, SB-CI8 column was used under the mobile phase of acetonitrile-1% Acetic acid（12 ： 88） at a flow of 1.0 mL,min-L The detection wavelength was 255 nm and the column temperature was maintained at 30 ℃. RESULTS The standard curve of eupalinolide A was linear in the range of 0.02-1.88 μg and eupalinolide B was linear in the range of 0.44-4.44 μg. The standard curve of hyperin was linear in the range of 0.101-1.414 μg. CONCLUSION This method was simple, accurate and reproducible for the determination of eupalinolide A, B and hyperin in Eupatorium lindleyanum DC.

关 键 词：野马追 野马追内酯A 野马追内酯B 金丝桃苷 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学]