羊睾丸提取液对铅损伤小鼠精子顶体形成的影响  

Effect of caprine testicular extract on acrosome formation of spermatids of lead-injured mice

作　　者：田洪艳[1] 李质馨[1] 王弘珺[1] 朱辛为[1] 潘晓燕[1] 刘忠平[1] 林冬静[1] 

机构地区：[1]吉林市吉林医药学院组织学与胚胎学教研室,吉林省132013

出　　处：《解放军医学杂志》2012年第11期1040-1043,共4页Medical Journal of Chinese People's Liberation Army

基　　金：吉林省教育厅"十二五"科技研究项目(2012485;2012484)~~

摘　　要：目的观察羊睾丸提取液对铅损伤小鼠精子顶体形成的影响。方法成年健康清洁级ICR雄性小鼠30只,随机分为对照组、模型组和给药组,每组10只。模型组采用醋酸铅(30mg/kg)灌胃建立睾丸损伤模型,给药组在醋酸铅灌胃的同时腹腔注射羊睾丸提取液0.5ml,对照组采用等体积蒸馏水灌胃及腹腔注射,每天1次,共21d。经心脏行甲醛灌流固定,制备睾丸标本,PAS染色,光镜下观察圆形精子细胞期顶体的形态学变化。结果与对照组相比,模型组精子顶体形成障碍,顶体囊泡不完整,膜皱缩,较粗糙,结构模糊;给药组精子顶体形成接近对照组,顶体囊泡形状较规则,膜平滑。结论羊睾丸提取液可保护小鼠精子顶体在形成过程中免受铅的损害,从而维持其正常的结构及功能。Objective To investigate the effect of caprine testicular extract on the acrosomal formation of spermatids of lead-treated mice, and find a theoretical and experimental basis for further research on the effects of caprine testicular extract on the reproductive system. Methods The study was carried out on thirty male adult ICR mice, which were randomly assigned into control group, model group and treatment group （10 each）. The testis-injury model was reproduced by intragastric administration of lead acetate （30mg/kg, 0.1ml/10g）. Animals in treatment group received intragastric administration of lead acetate and intraabdominal injection of 0.5ml caprine testicular extract at the same time for 21days, and the mice in control group received the same amount of distilled water by intragastric administration and 0.5ml distilled water by intraabdominal injection. By the end of the experimental period, the mice were sacrificed. The testes were fixed by cardiac perfusion and processed for light microscopic examination. The acrosomal formation in round spermatids was observed by PAS staining. Results Compared with the control group, the acrosomal formation in model group was blocked. Acrosomal vesicle was incomplete, the membrane was shrunken, rough and fuzzy. The acrosomal formation in treatment group was similar to that of the control group. The structure of the acrosomal vesicle was complete and the membrane was smooth. Conclusion Administration of caprine testicular extract may protect the acrosomal formation of spermatids from lead poisoning, and the normal structure and function of the acrosome are preserved. Thus, indepth study of the effects of caprine testicular extract may contribute to the treatment of male infertility caused by heavy metal-induced testicular damage, and offer an effective protective measure for the lead-exposed males.

关 键 词：睾丸提取物 铅中毒 精细胞 顶体 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]