高原驻训人员返回平原前后体内氧代谢和SHH信号通路的变化及意义  

Changes and significance of oxygen-metabolism and SHH signal pathway in soldiers trained in high altitude after returning to plains

作　　者：刘莉[1] 黄文豪 米玛扎西 周其全[3] 王关嵩[1] 王长征[1] 

机构地区：[1]第三军医大学新桥医院野战内科研究所,全军呼吸内科研究所,重庆400037 [2]解放军第八医院,西藏日喀则857000 [3]第三军医大学高原军事医学系高原疾病学教研室,国家教育部高原医学重点实验室,重庆400038

出　　处：《解放军医学杂志》2012年第11期1086-1090,共5页Medical Journal of Chinese People's Liberation Army

基　　金：国家科技支撑计划课题(2009BAI85B03)~~

摘　　要：目的研究平原部队官兵在高原驻训6个月后返回平原的过程中,体内氧代谢与SHH信号通路的变化情况。方法选择从平原（海拔200m）进入高原（海拔3960m）驻训的男性官兵80人,年龄20~30（22.3±2.9）岁,在高原驻训6个月后返回平原。在进驻高原前、高原居留6个月及返回平原后第2天,采集空腹静脉血标本,采用分光光度比色法检测血清超氧化物歧化酶（SOD）活性及丙二醛（MDA）的含量,采用ELISA法检测血清SHH的含量,实时定量PCR检测外周血白细胞中SHH mRNA的转录水平,Western blotting检测细胞内SMO和细胞核蛋白GLI2的表达。结果进驻高原后,血清SOD活性显著降低（P〈0.05）,MDA含量显著升高（P〈0.05）。外周血SHH蛋白和mRNA表达水平,以及外周血白细胞SMO和细胞核GLI2表达水平在高原驻训时显著高于进驻高原前和返回平原后,但返回平原后仍显著高于进驻高原之前（P〈0.01）。结论高原暴露对体内氧代谢及SHH信号通路具有明显影响,高原低氧环境是激活SHH信号通路的原因之一。Objective To observe the changes in oxygen metabolism and sonic hedgehog （SHH） signaling pathway in soldiers returning to plains after being stationed and trained for 6 months in a plateau. Methods Eighty male officers and soldiers, aged 20-30 （22.3 ± 2.9） years, after being stationed and trained on plateau （altitude 3960m） for 6 months and returned to plain region （altitude 200m）, were selected as subjects. Before their returning to plateau, 6 months after their station and training in plateau, and 2 days after their returning to plain, fasting venous blood samples were collected, the serum levels of superoxide dismutase （SOD）, malondialdehyde （MDA） and Sonic Hedgehog （SHH） were determined by ELISA, the transcription of SHH mRNA was assayed by RT-PCR, and the expressions of SMO and nucleoprotein GLI2 were detected by Western blotting. All the data mentioned above were collected for statistical analysis. Results As the subjects entered and garrisoned in plateau for 6 months, the activity of SOD decreased and the content of MDA increased significantly （P〈0.05）. Both the protein expression and mRNA transcription of SHH were significantly higher after staying in plateau than in plain. When they returned to plain, both parameters decreased significantly, but were still higher than that when they lived in plain （P〈0.01）. The expressions of SMO and nucleoprotein GLI2 showed a same tendency of changes. Conclusion High altitude environment may have a great influence on oxygen metabolism of organism and SHH signal pathway, and the hypoxic environment of high altitude region is one of the conditions in activating the SHH signal pathway.

关 键 词：高海拔 氧化性应激 猬蛋白质类 脱适应 

分 类 号：R135.6[医药卫生—劳动卫生]