人脂肪组织与骨髓来源间充质干细胞生物学特性的比较  

Comparison of biological characteristics of adipose-derived versus bone marrow-derived mesenchymal stem cells

作　　者：朱希山[1] 施薇[1] 台卫平[1] 任军[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院,100038

出　　处：《中华器官移植杂志》2012年第11期694-698,共5页Chinese Journal of Organ Transplantation

摘　　要：目的比较脂肪来源间充质干细胞（ADAS）和骨髓来源间充质干细胞（BMSC）的生物学特性。方法分离ADAS和BMSC，比较它们的表型、细胞倍增时间及分泌的相关因子，分别检测它们对T淋巴细胞的活化、细胞周期、增殖及凋亡的作用。结果BMSC和ADAS在细胞表型上类似，只有CD106的表达有差异；在增殖速率上，ADAS群体倍增时间为28h，显著高于BMSC的39h（P〈0．05）；ADAS和BMSC同样具有抑制T淋巴细胞增殖的能力，在有丝分裂原刺激和混合淋巴细胞反应的T淋巴细胞增殖中，这种抑制作用都具有剂量依赖性，在1：2时抑制作用极强，但是在1：100时这种抑制作用基本消失；在共培养时，ADAS和BMSC都可以使绝大多数的T淋巴细胞被抑制在G0／G1期，同时也可以抑制T淋巴细胞的早期活化，但是ADAS的上述作用均比BMSC弱；ADAS并不具有抑制T淋巴细胞凋亡的作用，而在TH0细胞向Tn1细胞或TH2细胞分化中所起的作用基本相似，主要抑制TH0细胞向Tn1细胞（表达IL-2和IFN-γ的T淋巴细胞）的分化，而对向TH2细胞（表达IL-4和IL-10的T淋巴细胞）分化没有明显的影响。结论ADAS与BMSC具有类似的免疫调节作用，在相同体积的脂肪组织中能够得到的干细胞前体细胞的数量是骨髓组织的10倍以上，因此ADAS较难以获得的BMSC更有广泛的应用前景。Objective To compare the biological characteristics of adipose-derived mesenchymal stem cells （ADAS） and bone marrow-derived mesenchymal stem cells （BMSCs）. Methods The adipose- and bone marrow-derived sources of mesenchymal stem cells were separated, and their phenotype, cell doubling time and the secretion of factors were compared. They were also used to detect T-cell cycle, activation, and proliferation inhibition- Results BMSCs and ADAS were similar on the cell phenotype and the differences only existed in the expression of only CD106. For the proliferation rate, ADAS grew faster than BMSCs （doubling time 28 h vs. 39 h, P〈0. 05） ; ADAS and BMSCs also had the same ability to inhibit T cell proliferation, and dose-dependent effects existed in mitogen-stimulated T-cell proliferation and MLR： there was a strong inhibitory effect in 1：2, but this effect disappeared at 1 ： 100. Both ADAS and BMSCs could arrest most T cells in the G0/G1 phase, but the role of ADAS was weaker than that of the BMSCs. ADAS could not inhibit apoptosis of T cells. ADAS and BMSCs played the same roles in inhibiting the differentiation of TH0 to Tn1 or TH2： mainly inhibiting differentiation of TH 0 to Ta 1 cells （IL-2- and IFN-γ-producing cells）, but having no significant effect on Tn2 cells （IL-4- and IL-10-producing cells）. Conclusion ADAS and BMSC have a similar role in immune regulation. In the same volume, fat tissue has the number of more than 10 times of stem cell precursor cells than that of bone marrow, so adipose tissue is a more promising stem ceils transplant source.

关 键 词：人 脂肪组织 骨髓 间质干细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]