氨溴索增强左氧氟沙星在铜绿假单胞菌生物被膜形成时的杀菌作用  

Ambroxol reinforce the bactericidal effect of levofloxacin on the Pseudomonas aeruginosa biofilm

作　　者：孔晋亮[1] 蔡双启[1] 陈一强[1] 闫萍[1] 张东伟[2] 简丽娟[1] 吴海英[1] 

机构地区：[1]广西医科大学第一附属医院呼吸内科,南宁530021 [2]广西柳州市人民医院呼吸内科

出　　处：《中华急诊医学杂志》2012年第11期1230-1233,共4页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金（30760084）;广西壮族自治区卫生厅基金课题（Z2010334）;广西壮族自治区中医药管理局基金课题（gzzell38）;广西壮族自治区教育厅课题（桂教201106LX092）;本研究获得广西大型仪器网测试补助

摘　　要：目的 观察盐酸氨溴索（AMB）对铜绿假单胞菌（P.a）的生物被摸（BF）破坏清除作用及其与左氧氟沙星（LFX）联合后对BF内P．a的影响。方法 分别建立PA01的3d和7d体外BF模型，随机（随机数字法）分为空白对照组、AMB组，AMB＋LFX组。AMB质量浓度分别为256μg／ml和512μg／ml。于体外早期和成熟BF模型形成后，分别加入不同质量浓度的AMB，作用24h后，连续稀释法行载体表面BF内P．a活菌计数，SEM观察载体表面BF形态学变化；采用单因素方差分析法（One-WayANOVA）进行计量资料的统计分析。结果 无论是早期BF还是成熟期BF，药物作用24h后SEM观察，AMB组载体表面BF较空白对照组明显减少，并且随着AMB质量浓度的增加，载体表面BF呈减少趋势。无论是早期BF还是成熟期BF，1μg／ml的LFX组可以减少BF内细菌（P〈0.05）；LFX和不同质量浓度的AMB联合后，BF内菌落计数较单独使用LFX组减少更明显（P〈0.05）；并且，LFX和512μg／ml AMB联合作用后，载体BF内菌落计数明显少于LFX和256μg／mlAMB联合组（P〈0.05）。结论AMB可以破坏已形成的早期和成熟BF；LFX单独作用可以部分减少载体BF内P．a，与AMB联合后表现出协同杀菌作用，且随着AMB质量浓度的增加协同杀菌作用更强。Objective To observe the destructive and scavenging effect of ambroxol （AMB） on the biofilm （BF） of Pseudomonas aeruginosa （P. a）. To evaluate the synergistically bactericidal effect of AMB along with levofloxacin （LFX） on BF of P. a. Methods The early model （cultured for 3 d） and mature model （cultured for 7 d） of P. a wild strain （PAO1） BF were established, in vitro, respectively. The models were randomly （random number） divided into control group, AMB group and AMB ＋ LFX group. The concentrations of AMB were 256 μg/ml and 512 /μg/ml, respectively. When the early BF model and mature BF model were made, different concentrations of AMB were added in AMB group and AMB ＋ LFX （ 1 μg/ml） was added in AMB ＋ LFX group. The number of viable P. a on the BF carrier was counted with the continuous dilution method 24 h after AMB or/and LFX added. Then, the BF morphological changes on the carrier surface were observed by using scanning electron microscopy （SEM）. Measured data were analyzed with single factor analysis of variance （ One-Way ANOVA）. Results Both in the early BF model and in the mature BF model, the SEM examination showed that the BF in AMB group was significantly reduced compared to the control group, and this reduction of BF was in dose-dependent manner. LFX 1 μg/ml could reduce the number of viable bacterial in BF in both early model and mature model （ P 〈 0. 05 ）. LFX with addition of different concentrations of AMB showed stronger bactericidal effect than LFX used alone identified by more significant reduction in the number of colony within the BF （ P 〈 0. 05 ）. Furthermore, the LFXcombined with 512 μg/ml AMB reduced more significant number of colony apparently than the LFX combined with 256 μg/ml AMB （P 〈 0. 05 ）. Conclusions AMB can destroy the early BF or mature BF partly, and LFX alone can partly reduce the number of viable P. a within BF. When LFX combined with AMB, they exert a synergistically bactericidal effect.

关 键 词：铜绿假单胞菌 生物被膜 盐酸氨溴索 左氧氟沙星 杀菌作用 

分 类 号：R378[医药卫生—病原生物学]