潜水人群铜绿假单胞菌基因多位点序列遗传分型  

Multilocus sequence typing scheme of Pseudomonas aeruginosa isolates from naval divers

作　　者：陈双红 陈锐勇 陈海庭 徐雄利 李慈 

机构地区：[1]海军医学研究所舰艇卫生研究室,高气压与潜水生理学全军重点实验室,上海200433

出　　处：《第二军医大学学报》2012年第11期1241-1244,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(81271796);上海市自然科学基金(09ZR1421000)~~

摘　　要：目的研究海军潜水人群铜绿假单胞菌分离株的遗传基因型,明确海军潜水人群铜绿假单胞菌携带株的流行特征。方法用多位点序列分型(multilocus sequence typing,MLST)技术、以铜绿假单胞菌7个管家基因acsA、aroE、guaA、mutL、nuoD、ppsA和trpE内部特定核酸片段作检测靶,对随机抽样海军潜水员携带的64株铜绿假单胞菌进行目的基因扩增和测序,应用Pseudomonas aeruginosa MLST数据库对测序结果进行分析,以获得检测靶基因的多位点序列信息;应用Bionumerics 4.0的LIAN,SplitsTree和eBURST分析程序对获得的MLST进行进一步的生物信息学分析,以获得流行株的遗传特征。结果 64株铜绿假单胞菌中53株可分为19个ST型,11株未能分型;其中ST274和ST260是优势克隆株,分别占18.75%(12/64)、15.62%(10/64)。结论海军潜水人群携带的铜绿假单胞菌具有遗传多样性和优势基因型,MLST分型对于研究海军潜水人群携带的铜绿假单胞菌遗传差异与流行特征具有重要意义。Objective To investigate the genetic phenotypes of Pseudomonas aeruginosa isolates from naval divers,so as to obtain the epidemic character of Pseudomonas aeruginosa isolates in different naval divers.Methods A total of 64 Pseudomonas aeruginosa strains from naval divers were sequenced by multilocus sequence typing（MLST） using 7 house-keeping genes： acsA,aroE,guaA,mutL,nuoD,ppsA and trpE.The sequencing results were analyzed based on Pseudomonas aeruginosa international MLST database to obtain the allelic profiles and sequence types（STs）.MLST data were analyzed by Bionumerics 4.0 （http：//pubmlst.org/mlstanalyse） using LIAN,SplitsTree and eBURST.Results Data from MLST revealed a high diversity among the strains.Out of the 64 strains,53 strains could be assigned to 19 STs and 11 could not be assigned.ST274 and ST260 were the dominant strains,with ST274 accounting for 18.75%（12/64） and ST260 accounting for 15.62%（10/64）.Conclusion Pseudomonas aeruginosa strains in naval divers have high diversity and preponderant genotypes.MLST is of great significance for Pseudomonas aeruginosa genomic study and epidemiology surveillance.

关 键 词：铜绿假单胞菌 多位点序列分型 基因型 潜水 

分 类 号：R378.991[医药卫生—病原生物学]