精原干细胞移植相关技术研究进展  

Research Progress of Spermatogonial Stem Cells Transplantation Technology

作　　者：刘玲[1] 朱化彬[1] 王琛[1,2] 郝海生[1] 赵学明[1] 冯荣[3] 杜卫华[1] 秦彤[1] 刘岩[1] 王栋[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室,北京100193 [2]广西大学动物科技学院,南宁530004 [3]乌兰察布职业学院,乌兰察布012000

出　　处：《畜牧兽医学报》2012年第11期1677-1682,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：“十二五”国家科技支撑计划课题(2011BAD19B02)

摘　　要：精原干细胞的分离培养及移植等相关技术对于雄性动物不育、精子发生机理的研究及转基因新技术的建立都具有重要意义。虽然小鼠精原干细胞的生物学特性已经研究得较为清晰,并建立了较为成熟的分离、纯化、培养技术体系,其受体制备和移植技术也较为成熟,但是,技术效率仍然很低。对小鼠精原干细胞生物学特性、分离培养及受体制备与移植方法的分析总结,将对干细胞制备最佳时机的确定、已有精原干细胞分离纯化、培养和受体准备及移植方法的改进提高起到重要的推动作用,也为今后大型动物的相关研究指明了方向。The culture and transplantation of spermatogonial stem cells（SSCs） and related resear- ches will be significant in some fields, such as male infertility, spermatogenesis mechanism research and set-up of transgenic technology. Biological characteristics of mouse SSCs had been studied clearly. The technology system of isolating, purifying and cultivating these stem cells had been built, as well as recipient preparation and transplantation technology. However, the effi- ciency of these technologies is not high. Reviewing all the above technologies, not only the opti- mum preparation time of the stem ceils can be determined, but also the technique of recipient preparation and the techniques of purification, cultivation and transplantation of SSCs will be improved. And these will also provide guidance for the future study on farm animals in this field.

关 键 词：精原干细胞 分离 纯化 培养 移植 

分 类 号：S813.3[农业科学—畜牧学]