妊娠后期胎儿宫内生长受限对蒙古绵羊胎儿胸腺生长发育的影响  

Effects of IUGR during Late Pregnancy on the Fetal Thymus Development of Mongolia Ovine

作　　者：张园[1] 高峰[1] 刘迎春[2] 傲丽格日玛[1] 

机构地区：[1]内蒙古农业大学动物科学学院,呼和浩特010018 [2]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《畜牧兽医学报》2012年第11期1710-1715,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然基金项目(30800788;31260559);内蒙古自然科学基金项目(2009BS0405);内蒙古自治区高等学校青年科技英才支持计划(NJYT-12-B09)

摘　　要：本试验旨在研究妊娠后期胎儿宫内生长受限(Intrauterine growth retardation,IUGR)对蒙古绵羊胎儿胸腺及其T淋巴细胞发育的影响。选择健康的蒙古绵羊42只(经同期发情受孕),在妊娠90d时选择6只母羊进行屠宰,其余按体质量随机分配到3个组:限制组1(RG1,0.175MJ.kgW-0.75.d-1(n=14))、限制组2(RG2,0.33MJ.kgW-0.75.d-1(n=12))和自由采食组(CG,0.67 MJ.kgW-0.75.d-1,(n=10)),进行不同能量水平饲养。至妊娠140d,各组再选择6只母羊进行屠宰。结果表明,妊娠后期限饲母羊严重限制了限饲组胎儿体质量(P<0.01),导致RG2(P<0.05)、RG1组(P<0.01)胎儿胸腺质量、胸腺皮质厚度、胸腺总DNA含量以及RG1组胎儿胸腺的皮质/髓质比值、总蛋白质含量显著低于CG组(P<0.01);RG1组胎儿胸腺的SOD酶活性(P<0.05)、T-AOC浓度(P<0.01)显著低于CG组,GSH-PX酶活性(P<0.05)、MDA浓度(P<0.05)显著高于CG组;RG2、RG1组胎儿的胸腺中CD4+CD8+双阳性T淋巴细胞均显著低于CG组(P<0.05)。结果显示,妊娠后期IUGR严重影响了胎儿胸腺及其T淋巴细胞的生长发育,这必将影响IUGR胎儿细胞免疫能力及出生后的健康。This study investigated the effects of IUGR （Intrauterine growth retardation） during late pregnancy on the fetal thymus and the T cell lymphocyte development of Mongolia ovine. 42 Mongolia ewes mated at a synchronized estrus were selected and among them 6 ewes were slaughtered at the beginning of the experiment and the remaining 36 animals were allocated to three different groups： restricted group1 （RG1, 0.175 MJ·kgW-0.75·d-1, n=14）, restricted group2 （RG2, 0.33 MJ·kgW-0.75·d-1, n=12） and control group （CG, ad libitum, 0.67 MJ·kgW-0.75·d-1, n=10）. At 140 d of gestation, 6 ewes in each group were slaughtered and their fetuses were removed. The results showed that the fetal weight in both RG1 and RG2 groups were significantly reduced（P〈0.01） by maternal under-nutrition during late pregnancy, which led to fetal thymus weight, thymus cortical thickness, thymus DNA contents in RG1（P〈0.01） and RG2（P〈0.05） groups, and thymus cortical/medullary ratio, protein contents in RG1（P〈0.01） were decreased compared to that in CG group. The activities of SOD（P〈0.05） and the contents of T-AOC（P〈0.01） of fetal thymus in RG1 group were decreased, while the activities of GSH-PX and the contents of MDA were increased compared to CG group（P〈0.05）. Additionally, the CD4＋CD8＋ T lymphocyte subsets of fetal thymus were lower in both RG2 and RG1 groups than that in CG group（P〈0.05）. These results indicate that the fetal thymus and T lymphocyte are affected seriously by IUGR during late pregnancy, which may influence fetal immunity capability and postnatal health.

关 键 词：胎儿宫内生长受限 胎儿 胸腺 T淋巴细胞 发育 

分 类 号：S826[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]