广东猪输血传播病毒流行病学调查及其主要抗原编码区进化分析  

Epidemiological Investigation and Antigen Phylogeny Analyses in Guangdong for Porcine Transfusion Transmitted Virus

作　　者：陈轶雯[1] 李中圣 白挨泉[3] 陈善真 李其昌 姚火春[1] 王贵平 

机构地区：[1]南京农业大学动物医学院,南京210095 [2]广东现代农业集团研究院有限公司,广州510630 [3]佛山科学技术学院,佛山528000

出　　处：《畜牧兽医学报》2012年第11期1767-1772,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：广东省科技厅项目(2011A090400011)

摘　　要：为了解广东省猪群中猪输血传播病毒(TTSuVs)的感染情况,采用Nest-PCR方法对广东省7个不同地区规模化猪场抽检采集的159份育肥猪血清进行检测。结果表明,TTSuVs的2个种——TTSuV1和TTSuV2的阳性检出率分别为54.72%和35.22%,二者的共感染率为29.56%,说明TTSuVs在广东猪群中有较为广泛的传播。挑选Nest-PCR检测TTSuV1和TTSuV2为阳性的样品,分别在病毒保守区设计引物,扩增病毒基因,测序验证后,与其它已发表的不同属种TTVs ORF1序列进行进化树分析。结果表明猪TTVs与人、犬、猫、猴的TTVs分别处在进化树的不同分枝,具有很大差异;本次研究获得的TTSuV1ORF1序列与国内多株已发表的TTSuV1ORF1序列相似性为72%～75%;获得的TTSuV2ORF1序列与现有测序的多株TTSuV2的ORF1序列相似性为88%～97%。此研究为进一步研究TTSuVs流行状况、血清学诊断方法和临床防治技术奠定基础。The objective of this study was to investigate the prevalence of porcine transfusion transmitted virus （TTV） species （TTSuV1 and TTSuV2）. The study was conducted in 7 different intensive pig farms of Guangdong province in China. A total of 159 swine sera were collected, and then the virus were tested using Nest-PCR. The results demonstrated that the TTSuV1 and TTSuV2 infection rates were 54.72% and 34.22% respectively, and the co-infection rate was 29.56%. We designed PCR primers based upon the conserved region of the two viruses for amplifying the two virogenes. By blast analysis with our cloned TTsuVs sequences in NCBI database, we found that our isolated TTsuV1 ORF1and TTsuV2 ORF1, have high homologousto to those known TTSuVs sequences,72%-75% and 88%-97% respectively; while have big gaps with the TTVs which infecting other species like feils, canis, tupaia, tamarin, douroucouli and homo in the nucleotide sequence. To sum up, TTSuVs had been widely distributed in Guangdong’s pig populations. This study laid the foundation for further research of the prevalence situation, serologic diagnostic methods and to prepare for safe and valid vaccine.

关 键 词：TTSuV1 TTSuV2 流行病学 抗原进化分析 

分 类 号：S852.659.2[农业科学—基础兽医学]