1株猪繁殖与呼吸综合征病毒ORF5基因缺失毒株的基因组特征与演化分析  

Genomic Characterization and Evolvement Analysis of a Porcine Reproductive and Respiratory Syndrome Virus Isolate with Deletion in GP5

作　　者：付向晶[1] 王丹阳[1] 王兴龙[1] 张渭东[1] 宋祥军[1] 刘阳坤[1] 唐文雅[1] 慕国辉[1] 刘海金[1] 贺生芳[1] 杜恩岐[1] 杨增岐[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2012年第11期1773-1779,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："十一五"国家科技支撑计划项目(2006BAD06A08)

摘　　要：为了解陕西猪场猪繁殖与呼吸综合征病毒(PRRSV)的变异情况,对发病猪场进行PRRSV分离,采用RT-PCR方法对分离株NSP2基因和ORF5基因分别进行扩增,测序后进行序列分析。结果显示:得到1株PRRSV,命名为HZ1007株,属于美洲型;其NSP2 481位和533—561位存在30个氨基酸的不连续缺失,与高致病性PRRSV分子特征相似;GP5 85—95位氨基酸处出现了11个氨基酸的连续缺失;系统进化分析发现HZ1007与国内高致病性代表毒株HUN4、JXwn06、SY0608等亲缘关系较近,且与HUN4株相似性最高,为97.4%。本研究发现了GP5缺失型PRRSV,这在国内外尚属首例,且HZ1007株与高致病性PRRSV亲缘关系较近,在系统进化分析树上与高致病性毒株同属一个分支,其可能是高致病毒株新的变异型,其致病性和生物学特性需进一步研究分析。In order to understand the variation of porcine reproductive and respiratory syndrome virus （PRRSV） in Shaanxi province, the virus was detected from swine clinical samples, NSP2 and ORF5 genes were amplified, cloned and sequenced for analyzing the genetic variation of PRRSV. The result showed that the strain belonged to the North American genotype, named HZ1007. It had discontinuous 30 amino acids deletion in the aa position of 481 and 533-561 of NSP2, which was the same deletions with the highly pathogenic PRRSV. Moreover, the ORF5 gene of HZ1007 strain contained a continuous 11 amino acids deletion in the aa positon of 85-95. PRRSV HZ1007 was highly homologous to HUN4 and SY0608 strains. Phylogenetic tree analysis revealed that HZ1007 had closer relationship with the highly pathogenic PRRSV than classical strains, it may be a new variant of the highly pathogenic strain. The athogenic force and biological characteristics needed further research and analysis.

关 键 词：猪繁殖与呼吸综合征病毒 NSP2基因 GP5基因缺失 序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学]