B亚群禽白血病病毒灭活疫苗的免疫效力分析  

Protective Effect Analysis of Inactivated Vaccine of Subgroup B Avian Leukosis Viruses in Chicken Breeder Farms

作　　者：李薛[1] 李卫华[1,2] 赵鹏[1] 崔治中[1] 王鑫[3] 董宣[1] 

机构地区：[1]山东农业大学动物科技学院山东省畜禽疫病防制工程技术研究中心,泰安271018 [2]中国动物卫生与流行病学中心,青岛266032 [3]中国农业科学院家禽研究所,扬州225003

出　　处：《畜牧兽医学报》2012年第11期1788-1794,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家公益性行业(农业)科研专项种鸡场禽白血病防控与净化技术的集成(#201203055)经费资助

摘　　要：本研究旨在制备B亚群禽白血病的灭活疫苗,探索能否通过种鸡群疫苗免疫减少带毒率,从而加快禽白血病净化。将B亚群禽白血病病毒SDAU09C2株在鸡胚成纤维细胞(CEF)上连续培养复制大量病毒,制备灭活疫苗,用该疫苗免疫9只产蛋祖代鸡,同时设未免疫种鸡对照。收取种蛋孵化,对孵出雏鸡接种SDAU09C2,检测和比较有和无母源抗体鸡的病毒血症、泄殖腔p27、抗体动态。该疫苗在所有经免疫的成年鸡(9/9)诱发抗体反应,在三免后第3周产生的抗体水平最高,ELISA检测S/P值在1.69～1.89(阳性临界值为0.4),且维持4周以上后仍未下降。经过疫苗免疫过的母鸡在其抗体水平最高的前后1周内收集种蛋进行孵化,其雏鸡含母源抗体阳性率的在70%(12/17)左右。雏鸡1日龄攻毒,含母源抗体的12只雏鸡中只有4只检出病毒血症,且持续时间都很短。所有9只不含母源抗体的雏鸡在攻毒后4～8周内全部出现病毒血症,而且在观察期内呈现持续性病毒血症。该疫苗能使全部产蛋祖代鸡产生较高水平的特异性抗体,为雏鸡提供母源抗体,有效预防或减缓ALV-B的早期感染。To develop a inactivated vaccine against subgroup B avian leukosis viruses （ALV-B） and determine if vaccination of chicken breeders could protect young chicks from ALV-B horizontal infection at early stage and accelerate eradication progress. Chicken embryo fibroblast （CEF） cells were inoculated with SDAU09C2 strain of ALV-B and ALV-CEF was inactivated for preparation of oil-adjuvant vaccine. Eggs were collected from un-vaccinated and 9 vaccinated great parent female chickens for incubation. One-day-old chicks were bled for testing their maternal antibodies to ALV-A/B and then inoculated with ALV-B. Viremia and cloaca p27 detection dynamics were tested and compared between chick groups with or without maternal antibody to ALV. In 3 weeks after the 3rd vaccination with the inactivated vaccine, all 9 vaccinated breeders developed high antibody titers against ALV-A/B with ELISA read values of 1.69-1.89 （the positive base line was 0.4） and kept at the high titers for at least another 4 weeks. Maternal antibody was detected in 70% （12/17） of chicks from breeders with high antibody titers to ALV-A/B. Only 4 of 12 chickens with maternal antibodies developed temporary viremia and no viremia was detected in the left 8 maternal antibody positive chickens during the whole 14 weeks after inoculation of ALV-B at 1 day of age. But the persistant viremia was detected in 2-8 weeks in all 9 maternal antibody negative chickens and the viremia persisted in the whole tested period of 14 weeks after inoculation of ALV-B. The inactivated ALV-B vaccine could induce high titer antibody reaction to ALV-B, it could provide matarnal antibodies to 1-day-old chickens and protect chickens from early infection of ALV-B.

关 键 词：B亚群禽白血病病毒 细胞灭活疫苗 免疫保护 

分 类 号：S852.659.3[农业科学—基础兽医学]