低钙日粮添加依普黄酮对笼养蛋鸡骨组织显微结构和形态计量学的影响  

Effects of Adding Ipriflavone to Low-calcium Diet on Microstructure and Histomorphometry of Bone in Cage Layers

作　　者：吕文亭[1] 马利芹[1] 杨永红[1] 王鹏[1] 利凯[1] 

机构地区：[1]河北北方学院动物科技学院,张家口075131

出　　处：《畜牧兽医学报》2012年第11期1818-1824,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河北省自然基金(C2010001447);河北教育厅项目(2008410)

摘　　要：本试验旨在研究低钙日粮中添加依普黄酮对笼养蛋鸡骨质疏松症病鸡骨组织显微结构和形态计量学的影响。200只24周龄健康海兰褐蛋鸡随机分为4组:对照组(基础日粮中含钙3.55%)、低钙组(日粮中含钙1.27%)、试验Ⅰ组(8mg.kg-1依普黄酮+低钙日粮)和试验Ⅱ组(20mg.kg-1依普黄酮+低钙日粮),试验期60d。试验结束时,每组剖杀10只鸡,取左胫骨中段脱钙骨切片在OLYMPUS BX41型金相显微镜下观察骨组织形态结构的变化,并以Image-Pro Plus 6.0图像分析软件测定骨组织形态计量学的相关静态参数。结果表明:骨显微结构观察,与对照组相比,低钙组蛋鸡胫骨皮质骨出现许多吸收腔,在吸收腔内有大量破骨细胞,髓质骨骨小梁表面破骨细胞增多,骨吸收大于骨形成,导致骨质疏松;试验Ⅰ组蛋鸡胫骨皮质骨有吸收腔,吸收腔内和小梁表面可见较多破骨细胞,骨吸收未受抑制;试验Ⅱ组蛋鸡胫骨皮质骨外表面可见大量骨原细胞和成骨细胞,骨形成增强。形态计量学静态参数测定,与低钙组相比,试验Ⅰ、Ⅱ组的骨小梁面积、骨小梁面积百分比均极显著增加(P<0.01);试验Ⅰ、Ⅱ组的骨小梁周长、骨小梁数量高于低钙组,但差异不显著(P>0.05);试验Ⅱ组的骨小梁分离度显著降低(P<0.05)及骨小梁厚度显著增加(P<0.05)。上述结果说明,在低钙日粮中添加20mg.kg-1依普黄酮可使骨重建活动加强,改善笼养蛋鸡的骨代谢及缓解骨质疏松症的发生。This experiment was conducted to study the effects of adding Ipriflavone to low-calcium diet on microstructure and histomorphometry of bone in hyline brown laying hens. Two hundred 24-week-old hyline brown laying hens were randomly divided into four groups and fed on diets as follows： control group （containing calcium 3.55% in basic diet）, low calcium group （containing calcium 1.27% in low-calcium diet）, test groupⅠ（adding 8 mg·kg-1 Ipriflavone to low-calcium diet） and test groupⅡ（adding 20 mg·kg-1 Ipriflavone to low-calcium diet） for 60 days. At the end of experiment, 10 hens in each group were killed. Then, demineralized bone sections of the left middle tibias were made for observing the changes of bone microstructure by using OLYMPUS BX41 metalloscope, and some static parameters of bone histomorphometry were measured by Image-Pro Plus 6.0. Tibia microstructure was observed. Compared with control group, cortical bone of low calcium group had many resorption cavities, there were a large number of osteoclasts in the resorption cavities, and osteoclasts increased in the surface of medullary bone trabeculas, the effect of bone resorption was more than that of bone formation and led to osteoporosis. Cortical bone of test groupⅠ had many resorption cavities, there were more osteoclasts in the resorption cavities and the surface of medullary bone trabeculas, the effect of bone resorption was not inhibited. There were a large number of osteogenic cells and osteoblasts in the outer surface of cortical bone in test groupⅡ, the effect of bone formation was increased. And, Static parameters of bone histomorphometry were measured. Compared with low calcium group, Tb.Ar and %Tb.Ar in test group Ⅰ and Ⅱ were very significantly increased （P〈0.01）. Tb.Pm and Tb.N were higher in test group Ⅰ and Ⅱ than in low calcium group, but the differences were not significant （P〉0.05）. In test group Ⅱ Tb.Sp was significantly decreased （P〈0.05） and Tb.Th was significantly increased （P

关 键 词：笼养蛋鸡骨质疏松症 依普黄酮 低钙 骨显微结构 骨形态计量学 

分 类 号：S852.16[农业科学—基础兽医学] S852.31[农业科学—兽医学]