IFNα通过p204抑制大鼠主动脉外膜成纤维细胞增殖  

IFNα suppresses proliferation of rat vascular adventitial fibroblast via p204

作　　者：宋方[1,2] 吴强[1,2] 谭洪文 龙向淑[2] 袁军[3] 杨永耀[1,2] 

机构地区：[1]贵州省人民医院贵州省心血管病研究所,贵州贵阳550002 [2]贵州省人民医院心血管内科,贵州贵阳550002 [3]贵州省人民医院中心实验室,贵州贵阳550002

出　　处：《基础医学与临床》2012年第12期1431-1436,共6页Basic and Clinical Medicine

基　　金：贵州省优秀科技教育人才省长资金(黔省专合字2009-30)

摘　　要：目的研究干扰素诱导蛋白p204在大鼠主动脉血管外膜成纤维细胞(VAF)的基础表达,α干扰素(IFNα)对VAF细胞增殖及p204表达的影响。方法用免疫组织化学及免疫细胞化学观察p204蛋白的表达及分布;用West-ern blot及RT-PCR检测p204蛋白及Ifi204 mRNA表达;用MTT法检测VAF细胞增殖。结果 p204在大鼠主动脉血管外膜染色阳性,p204在鼠VAF细胞同时表达于胞质及胞核,以胞核表达更明显。应用1×106IU/L的IFNα刺激VAF细胞6 h以上即可诱导Ifi204 mRNA大量表达,IFNα作用24 h以上时p204蛋白大量表达。IFNα呈浓度依赖性抑制VAF细胞增殖,A value值由对照组的0.727±0.090逐渐降至4×106IU/L IFNα时的0.652±0.066及8×106IU/L IFNα时的0.587±0.043,伴随着p204表达相应增加(P<0.05)。结论 p204在大鼠主动脉VAF存在基础表达,其在VAF上表达并不局限于胞核,易被IFNα诱导其mRNA及蛋白表达上调;IFNα抑制VAF细胞增殖的作用可能部分与其诱导p204表达有关。Objective To study the basic expression of the interferon-inducible protein p204 in rat aortic vascular adventitial fibroblasts （VAFs）, and the effect of interferon alpha （IFNct） on VAF proliferation and p204 expression. Methods Using immunohistochemistry and immunocytochemistry to observe the expression and distribution of protein p204, using RT-PCR and Western blot to detect the expression of induced p204 mRNA or protein. After treatment with different concentrations of IFNα, the cell proliferation of the VAF was observed by MTT assay and the corresponding p204 expression was detected by Western blot. Results p204 stain was positive on rat aortic ad- ventitia, in the cytoplasm and also nucleus of VAF, but its expression was more obvious in the nucleus. IFNct （the terminal concentration of 1×10^6 IU/L） stimulating VAF more than 6 hours was enough to induce Ifi204 mRNA significant expression, and more than 24 hours for enough expression of protein p204. IFNα inhibited cell proliferation of VAF was dose dependence, followed by p204 expression increased correspondingly （ P 〈 0. 05 ）. Conclusions There is basic expression of p204 in rat aortic VAF, p204 expression is not limited to the nucleus and IFNα can partly relate to the increased p204 expression.

关 键 词：干扰素 干扰素诱导蛋白 P204 成纤维细胞 增殖 

分 类 号：R543[医药卫生—心血管疾病]