外源生长激素对尼罗罗非鱼生长、骨骼肌纤维增生及肥大的影响  

Effect of Exogenous Growth Hormone on Growth and Skeletal Muscle Hyperplasia and Hypertrophy of Nile Tilapia(Oreochromis niloticus)

作　　者：苗田田[1] 赵金良[1] 苌建菊[1] 许淼洋[1] 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306

出　　处：《农业生物技术学报》2012年第11期1315-1320,共6页Journal of Agricultural Biotechnology

基　　金：现代农业产业技术体系专项资金(No.CARS-49-4B)

摘　　要：生长激素是影响动物生长发育的最重要内分泌激素,为了探讨外源生长激素对尼罗罗非鱼(Oreochromis niloticus)生长、骨骼肌纤维增生以及肥大的影响,本实验对同一批次尼罗罗非鱼幼鱼进行为期7周的外源重组人生长激素(hGH)肌肉注射,并设置了磷酸盐缓冲液(PBS)注射对照组,每周注射1次,测量体重、体长等表型生长指标,同时,隔两周采集背部肌肉,利用石蜡切片技术分析骨骼肌背侧第一肌节中肌纤维数目与面积的变化。结果显示:(1)注射后第1周起,实验组鱼的体重、体长即高于对照组,但差异不显著(P>0.05);第5周起,实验组鱼体重显著高于对照组鱼(P<0.05),第7周起,实验组鱼体长极显著高于对照组鱼(P<0.01)。(2)注射后第1周起,实验组鱼肌纤维数目、总面积均高于对照组鱼,差异不显著(P>0.05);第5周起,实验组鱼肌纤维的外源性增生和肥大现象极显著(P<0.01)。研究结果表明,外源生长激素对尼罗罗非鱼的表型生长、骨骼肌肌纤维的增生与肥大均有显著促进作用,肌肉生长中以肥大生长更为明显。Growth hormone is one of the most important endocrine hormones that can affect animal growth. In order to explore the influence of exogenous growth hormone on growth, skeletal muscle hyperplasia and hypertrophy of Nile tilapia （Oreochromis niloticus）, the same batch of young fish were seperated into two groups： The experimantal group which was injected with exogenous recombinant human growth hormone （hGH） in muscle once a week, and the control group which was injected with phosphate buffer（PBS） only. The body length and weight of the fish were measured weekly, skeletal muscle fibers number and area in the first dosal myomere were analysed by means of paraffin tissue sections once every two weeks. The results showed that： （1） Body length and weight of the experimental group were bigger than that of the control group since the first week, but the difference was not significant （P〉0.05）. however, significant variation were observed from the 5th and 7th week for body weight and body length（P〈0.01 or P〈0.05）, respectively. （2） Fibers number and area in the experimantal group were also bigger than that in the control group since the first week, exogenous hyperplasia and hypertrophy were detected obviously in the experimental group from the 5th week （P〈0.01）. These results suggested that exogenous growth hormone can promote growth in body length, body weight, as well as in skeletal muscle, hypertrophy growth is more dominant than hyperplasia growth in skeletal muscle growth of Nile tilapia.

关 键 词：外源生长激素 表型生长 肌肉生长 尼罗罗非鱼 

分 类 号：S828.5[农业科学—畜牧学]