肺炎链球菌感染猕猴肺及气管内分泌型免疫球蛋白A(sIgA)的表达  

Expression of Secretory Immunoglobulin A(sIgA) Protein and mRNA in Lung and Trachea of Macaque(Macaca mulatta) Infected Spontaneously Strpetococcus pneumoniae

作　　者：章瑶[1] 罗启慧[1,2] 陈恋[1] 陈正礼[1,2] 程安春[1,2,3] 曾文[2] 

机构地区：[1]四川农业大学动物疫病与人类健康四川省重点实验室,雅安625014 [2]四川农业大学实验动物工程技术中心国家实验用猕猴种源基地,雅安625014 [3]四川农业大学预防兽医研究所,成都611130

出　　处：《农业生物技术学报》2012年第11期1321-1326,共6页Journal of Agricultural Biotechnology

基　　金：四川省科技厅资助项目(No.2011JY0054);教育部"长江学者和创新团队发展计划"团队资助项目(No.IRT0848);四川省国际科技合作与交流研究计划项目(No.2010HH0013);四川省教育厅重点项目(No.09XZ006)

摘　　要：肺炎链球菌(Streptococcus pneumoniae)感染一直是影响猕猴(Macaca mulatta)健康的重要病菌。为了研究自发性肺炎链球菌感染猕猴肺脏及气管内分泌性免疫球蛋白A(sIgA)蛋白及mRNA的表达与分布情况,本实验采用免疫组织化学技术和组织原位杂交技术进行检测。结果表明,感染组sIgA蛋白产物在肺泡隔内的表达量高于健康组且差异极显著(P<0.01),肺脏血管壁内表达量高于健康组且差异显著(P<0.05),气管上皮层及固有层内OD值高于健康组差异显著(P<0.05),上皮层中阳性表达面积高于健康组差异显著(P<0.05)。sIgA mRNA在肺泡隔、肺脏血管内及血管壁的阳性表达量远高于与健康组,且差异均极显著(P<0.01),气管上皮层及气管肌肉层中OD值高于健康组且差异极显著(P<0.01),固有层中OD值高于健康组且差异显著(P<0.05);sIgA mRNA在气管上皮层中的表达面积与健康组相比差异显著(P<0.05)。结果证实肺炎链球菌感染猕猴肺脏与气管中有大量的sIgA参与了免疫与调控的过程。研究结果为该疾病的定向治疗及免疫途径的确立提供新思路。Streptococcus pneumoniae is the importance germ to influence macaques＇s health. In order to investigate the expression and distribution of secretory immunoglobulin A （sIgA） protein and mRNA in lung and trachea of macaque infected Streptococcus pneumoniae spontaneously, immunohistochemistry and in situ hybridization were used. Results showed that the positive area of sIgA protein in alveolar septum were extremely higher than that of normal group （P〈0.01）, while in pulmonary vascular wall the difference were significantly higher （P〈0.05）, the optical density and positive area of slgA in tracheal epithelium and lamina propria were higher than that of normal group （P〈0.05）. The expression of slgA mRNA in alveolar septum, pulmonary intravascular and vessel wall were extremely higher than that of normal group （P〈0.01）, while in tracheal epithelium was significantly higher （P〈0.05）; compared with normal group, the optical density in tracheal epithelium and muscular layer were significantly higher than that of normal group （P〈0.01）, and it was higher in lamina propria of trachea （P〈0.05）. It can be concluded that slgA participated the immunomodulation process of Streptococcus pneumoniae infection in lung and trachea. The results give us a new ideas to establish directional treatment and immune way.

关 键 词：肺炎链球菌 分泌性免疫球蛋白A(sIgA) 免疫组化 组织原位杂交 猕猴 

分 类 号：S858.28[农业科学—临床兽医学]