盾叶薯蓣胚乳再生体系的建立及其染色体倍性鉴定  

A Regeneration System and Ploidy Identification of Plantlets for Endosperm of Dioscorea zingiberensis

作　　者：李明军[1,2] 郭婧[1] 李翔[1] 李纪强[1] 王医鹏[1] 张晓丽[1,2] 刘永康 

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]河南省高校道地中药材保育及利用工程技术研究中心,新乡453007 [3]温县农业科学研究所,温县454881

出　　处：《植物学报》2012年第6期654-660,共7页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.81274019);河南省科技创新杰出人才计划(No.114200510013)

摘　　要：以盾叶薯蓣(Dioscorea zingiberensis)的胚乳为外植体,研究了不同植物生长调节剂对胚乳愈伤组织诱导及植株再生的影响,并鉴定了再生植株。结果表明:愈伤组织诱导形成的适宜培养基为MS+2.0mg·L–12,4-D+0.5mg·L–16-BA,不定芽分化的适宜培养基为MS+2.0mg·L–16-BA+0.1mg·L–1NAA,生根的适宜培养基为1/2MS+0.3mg·L–1NAA;再生植株炼苗移栽后,成活率可达80%;对获得的再生植株腋芽生长点进行染色体制片观察,发现染色体数目为20的细胞占观察细胞总数的10%,染色体数目为21–29的细胞占16%,染色体数为30的细胞占74%;获得了三倍体植株。We studied the effect of various plant growth regulators on inducing endosperm callus and plant regeneration using the endosperm of Dioscorea zingiberensis as explants and identified the ploidy of plantlets. The optimal medium for inducing callus, adventitious bud differentiation and root formation was MS＋2.0 mg.L^–12,4-D＋0.5 mg.L^–16-BA, MS＋2.0 mg.L^–16-BA＋0.1 mg-L^–1NAA and 1/2MS＋0.3 mg-L^–1NAA, respectively. On observing the growth points of axillary buds in regenerated plants by chromosome slice production techniques, 10%, 16% and 74% of total observed cells had 20, 21-29 and 30 chromosomes, respectively. We obtained triploid plantlets.

关 键 词：染色体鉴定 盾叶薯蓣 胚乳培养 再生植株 三倍体 

分 类 号：S567.239[农业科学—中草药栽培]