雏番鸭胰腺型鸭1型甲肝病毒分离鉴定及VP1基因分析  

Identification and Sequence Analysis of Duck Hepatitis A Virus Type 1 Isolated from Muscovy Duckling with Pancreatitis

作　　者：傅光华[1] 陈红梅[1] 黄瑜[1] 施少华[1] 彭春香[1] 江斌[1] 程龙飞[1] 万春和[1] 傅秋玲[1] 林建生[1] 林芳[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013

出　　处：《福建农业学报》2012年第9期945-950,共6页Fujian Journal of Agricultural Sciences

基　　金：现代农业产业技术体系建设专项资金(CARS-43);福建省自然科学基金(2011J01113);福建省种业创新专项(2011FJZY-9);福建省农业科学院科技下乡"双百"行动项目(SBMX1224)

摘　　要：采集自胰腺发黄或出血的雏番鸭病料经RT-PCR检测为鸭1型甲肝病毒阳性后,接种11日龄非免疫番鸭胚获得一株病毒(命名为MPZJ1206)。该株病毒在不同温度下均不凝集鸡、鸭和绵羊红细胞;鸭胚中和试验表明蚀斑纯化毒可被经典的鸭1型甲肝病毒高免血清特异性所中和。该株病毒对7日龄雏番鸭的致死率为38.5%,病死鸭剖检病变与临床发病鸭相同,且从病死鸭脏器中回收分离到的病毒经鉴定仍为鸭1型甲肝病毒。应用鸭1型甲肝病毒VP1基因特异性引物从该株病毒克隆获得的714bp的基因片段,与经典鸭1型甲肝病毒分离株Du/CH/LGD/111239VP1基因的同源性最高,为99.1%,经遗传进化分析表明该株病毒属鸭1型甲肝病毒谱系。以上结果表明,雏番鸭胰腺炎是由鸭1型甲肝病毒感染所致,这一新病型明显不同于经典的鸭1型甲肝病毒感染引起的肝脏出血,鉴于此,建议将本试验分离株命名为胰腺型鸭1型甲肝病毒。Samples came from Muscovy ducklings with yellowed or hemorrhagic pancreatitis were positive for duck hepatitis A virus-1 （DHAV-1） by RT-PCR. A virus strain （named as MPZJ1206） was abtained from Virus- containing allantoic fluid after inoculation of embryonated Muscovy duck eggs with DHAV-1-- positive samples. The isolate shows no hemagglutinating activity with chickens, ducks or sheep red blood cells at different temperature. Plaque purified virus was specifically neutralized by anti-DHAV-1 hyperimmune serum. Seven-day-old Muscovy ducklings inoculated with the virus showed a mortality rate of 38.5%, and yielded the same pathological characteristics as those of clinical onset duck had. The virus strain also recovered from the pancreas of Muscovy duckling died in the trials. A 714-bp fregment gene was abtained by using a set of primers aimed at VP10RF gene, and shared 99. 1% nucleotide sequence identity with that of classical DHAV-1 strain Du/CH/LGD/111239. Phylogenetic analysis showed that MPZJ1206 strain lay in the viral lineage of DHAV-1. The above results indicate that pancreatitis in Muscovy ducklings was caused due to the infection of duck hepatitis A virus type 1, and this pathotype is distincted from hemorrhagic hepatitis caused by classical duck hepatitis A virus, so it advised that the virus isolated in this study could be named as pancreatitis-type DHAV-1.

关 键 词：鸭甲肝病毒 胰腺型 雏番鸭 VP1衣壳蛋白 

分 类 号：S852[农业科学—基础兽医学]