烟草糖基转移酶基因SM-Ngt1在拟南芥中抗菌核病的初步研究  

Preliminary study on the resistance to Sclerotinia sclerotiorum in Aarabidopsis by a glycosyltransferase gene SM-Ngt1 from tobacco

作　　者：秦汉花[1] 谭艳平[1] 王春台[1] 刘学群[1] 

机构地区：[1]中南民族大学生命科学学院/生物技术国家民委重点实验室,武汉430074

出　　处：《华中农业大学学报》2012年第6期668-671,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31170296)

摘　　要：通过对转糖基转移酶基因SM-Ngt1的拟南芥T2代阳性植株接种油菜菌核病菌,鉴定其对菌核病的抗性,并应用实时荧光定量PCR(RT-PCR)确定植株中SM-Ngt1的表达水平。结果表明,拟南芥中SM-Ngt1基因对菌核病具有一定的抗性,且抗性的高低与体内SM-Ngt1的表达量具有明显的一致性。The resistance to Sclerotinia sclerotiorum of the transgenic Arabidopsis T2-positive plant with glycosyl transferase gene SM-Ngt1 from tobacco was identified by inoculating of Sclerotinia sclerotiorum pathogen.The levels of expression of SM-Ngt1 in the transgenic Arabidopsis T2-positive was determined through real-time fluorescence quantitative PCR（RT-PCR）.The results showed that SM-Ngt1 was resistant to sclerotium disease in Arabidopsis and the level of resistance was obviously consisted with the expression of SM-Ngt1 in the transgenic Arabidopsis.

关 键 词：SM-Ngt1 菌核病菌 RT-PCR 转基因 抗性 烟草 

分 类 号：Q78[生物学—分子生物学]