发根农杆菌诱导大豆毛状根体系的建立  

Establishment of soybean hairy root system induced by Agrobacterium rhizogenes

作　　者：宗晓秋[1] 张东升[1] 黄文坤[1] 彭焕[1] 彭德良[1] 

机构地区：[1]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193

出　　处：《华中农业大学学报》2012年第6期699-703,共5页Journal of Huazhong Agricultural University

基　　金：国家公益性行业(农业)科研专项(200903040)

摘　　要：选取中国大豆品种中黄13、黑豆44和黑豆51的子叶为外植体材料,用发根农杆菌(Agrobacterium rhizogenes)K599菌株诱导3个大豆品种产生毛状根,通过β-葡聚糖苷酶(GUS)染色进行转化效率检测。结果表明:在25℃、12h/d黑暗培养条件下,黑豆44上诱导的毛状根数量最多,显著多于中黄13和黑豆51;黑豆44诱导的毛状根转化率最高,显著高于中黄13,但与黑豆51没有显著差异;光照或黑暗培养对中黄13产生毛状根的效率没有显著影响,但黑豆44和黑豆51在黑暗培养条件下子叶产生的毛状根数明显高于光照培养条件下产生的毛状根数;大豆孢囊线虫(Heterodera glycines Ichinohe)能侵染3个大豆品种的毛状根,其中对黑豆51的侵染效率显著高于黑豆44和中黄13,侵染位点主要位于根系的生长点。Seedling cotyledons of the soybean cyst nematode（Heterodera glycines Ichinohe）-susceptible cultivars of Zhonghuang 13,Heidou 44 and Heidou 51 were co-cultured with Agrobacterium rhizogenes strain K599 under dark conditions.Transformation efficiency was tested through GUS staining.The results showed that there were significant differences among soybean genotypes in their susceptibility to A.rhizogenes.The conditions of 25 ℃ and 12 h/d light were found to be optimal for hairy root induction of Heidou 44.The transformation efficiency of Heidou 44 had no significant difference with that of Heidou 51,while it was significantly higher than that of Zhonghuang 13.Light or darkness had no effect on Zhonghuang 13,but the inducing efficiency of Heidou 44 and Heidou 51 in the dark conditions were significantly higher than that in light condition.The infective efficiency of Heidou 44 was significantly higher than that of Heidou 51 and Zhonghuang 13.

关 键 词：大豆 毛状根 大豆孢囊线虫 发根农杆菌 

分 类 号：S432.45[农业科学—植物病理学]