IL-18在妊娠早期大鼠下丘脑视前区核团的表达  

Expression of IL-18 in Nuclear Hypothalamus-area of Early Pregnant Rats

作　　者：杜秋婉[1] 司丽芳[1] 顾琨涛[1] 赵鹏[1] 陈树林[2] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003 [2]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《动物医学进展》2012年第11期66-69,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30972151);河南科技大学大学生研究训练计划(SRTP)项目(2012248);河南科技大学博士研究生科研启动基金项目(09001543)

摘　　要：为系统研究IL-18在胚泡植入前后期大鼠下丘脑中的表达,分别对胚泡植入前后期妊娠大鼠进行取材,固定后用免疫组化SP法,图像分析法进行研究。结果表明,IL-18在胚泡植入前后期大鼠下丘脑均有表达,主要表达于下丘脑视前室周核、视前大细胞核、视上核、视前内侧核、弓状核、视交叉上核、视前外侧核。与植入前期相比,植入后期妊娠大鼠下丘脑视前室周核、视前大细胞核、视上核、视前内侧核、弓状核、视交叉上核、视前外侧核的表达显著高于植入前期(P<0.05),下丘脑视前外侧核的表达极显著高于植入前期(P<0.01)。IL-18与胚泡植入密切相关,可能是IL-18通过下丘脑的反馈途径参与了妊娠调控,其表达变化与妊娠的建立和维持有关,在妊娠中起重要作用。Thi ultrasensitive immunohistochemistry SP method and image analysis were used to investigate the expression of interleukin-18（IL-18） in fixed rat hypothalamus in pre-implantation period and post-implantation period.The results showed that IL-18 expressed in rat hypothalamus in both pre-implantation period and post-implantation period,mainly expressed in the hypothalamic periventricular nucleus,supraoptic nucleus,preoptic large nucleus,medial preoptic nucleus,lateral preoptic nucleus,suprachiasmatic nucleus,arcuate nucleus.Compared with the pre-implantation period,the expression of IL-18 in post-implantation period in hypothalamic periventricular nucleus,supraoptic nucleus,preoptic large nucleus,medial preoptic nucleus,suprachiasmatic nucleus,arcuate nucleus was significantly higher（P0.05）,and extremely significant in hypothalamic preoptic lateral nucleus（P0.01）.The expression of IL-18 was related to establishing and maintaining pregnancy as the expression of IL-18 was closely related to blastocyst implantation,and IL-18 may be participated in regulating rat pregnancy through the endocrine system of hypothalamus.

关 键 词：下丘脑 IL-18 胚泡植入 

分 类 号：S852.16[农业科学—基础兽医学]