黑穗醋栗‘寒丰’离体快繁影响因素研究  

Study on the Factors Influencing Rapid Propagation in vitro of Ribes nigrum L.'Hanfeng'

作　　者：李金英[1] 赵春莉[1] 张志东[1] 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118

出　　处：《北方园艺》2012年第22期119-121,共3页Northern Horticulture

基　　金：吉林省教育厅资助项目(吉教科合字[2011]第45号)

摘　　要：以黑穗醋栗‘寒丰’植株不同组织部位为外植体,研究分析了离体快繁过程中的一些影响因素。结果表明:‘寒丰’茎尖消毒时间以0.1%升汞(HgCl2)消毒7min,带腋芽的茎段9min为宜,嫩茎以手摇消毒效果较好,其它辅助材料以0.1%升汞+2滴吐温消毒8min效果较佳;‘寒丰’组培苗在MS+BA 1.0mg/L+IBA 0.1mg/L+GA31.0～2.0mg/L培养基上能分化出大量丛生芽,且长势较好。Taking different part of Ribes nigrum L.＇Hanfeng＇ as materials,the factors that influencing culture in vitro of ＇Hanfeng＇ were studied by in vitro culture of different parts of organs,including stem tip and stem with auxiliary bud.The results showed that the optimal sterilization time of stem tip was 7 minutes with 0.1% HgCl2 and 9 minutes of auxiliary bud,tender stem with hand shelce had the better sterilize effect,0.1 HgCl2 plus two drops of Twain 8 min had betler effect.The cluster buds could be induced in MS＋BA 1.0 mg/L＋IBA 0.1 mg/L＋GA3 1.0~2.0 mg/L,and the plants grew well.

关 键 词：黑穗醋栗 '寒丰’ 离体快繁 

分 类 号：S663.903.6[农业科学—果树学]