傅里叶变换红外光谱对枯草芽孢杆菌的光学特性研究  

Optical Properties Research of Bacillus Subtilis Spores by Fourier Transform Infrared Spectroscopy

作　　者：冯明春[1] 徐亮[1] 高闽光[1] 焦洋[1] 魏秀丽[1] 金岭[1] 程巳阳[1] 李相贤[1] 冯书香[1] 

机构地区：[1]中国科学院安徽光学精密机械研究所,中国科学院环境光学与技术重点实验室,安徽合肥230031

出　　处：《光谱学与光谱分析》2012年第12期3193-3196,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(40905011;41105022);中国科学院知识创新工程重要方向项目(Y022091131)资助

摘　　要：使用傅里叶变换红外光谱(FTIR)技术,测量了两种不同浓度的枯草芽孢杆菌的红外透过率谱,根据朗伯-比尔定律计算出它们的质量消光截面,通过算出复折射率的虚部,再使用KK(Kramers-Kronig)关系,导出复折射率的实部,并对实验结果作了分析和讨论。通过研究枯草芽孢杆菌复折射率的测量和分析方法,对于进一步研究生物气溶胶的吸收和散射特性、拓宽生物气溶胶的测量和遥测技术方法,具有重要的意义。The authors measured IR transmission spectra of two different concentrations of bacillus subtilis spores by using Fou- rier transform infrared spectroscopy （F＇FIR） technology. The mass extinction cross section k of bacillus subtilis spores was cal- culated according to Lambert-Beer law and the imaginary part ni of the complex refractive index was also calculated through k. The real part nr of the complex refractive index was derived from the KK （Kramers-Kronig） relationship and the experimental results were also analyzed and discussed with the study of measurement and analysis method of the complex refractive index on bacillus subtilis spores, it is of great significance to further research the absorption and scattering characteristics, and to broaden t and remote sensing technology method of the biological aerosols.

关 键 词：傅里叶变换红外光谱 枯草芽孢杆菌 光学特性 

分 类 号：O657.3[理学—分析化学]