牛奶水溶液的偏振三维荧光光谱研究  

Research on Polarized Three-Dimensional Fluorescence Spectra of Milk Solution

作　　者：兰秀风[1] 顾春峰[1] 于银山[1] 卢礼萍[2] 

机构地区：[1]南京航空航天大学理学院,江苏南京210016 [2]南京农业大学理学院,江苏南京210095

出　　处：《光谱学与光谱分析》2012年第12期3281-3284,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金青年基金项目(11004103);中央高校基本科研业务费专项资金项目(NS2012062)资助

摘　　要：测得由伊利、蒙牛公司生产的纯牛奶、高钙奶、高钙低脂奶的偏振三维荧光光谱,发现在激发偏振片和发射偏振片取向平行时,偏振荧光强度高于取向垂直时的值。通过一个简单的模型估算了各种牛奶样品等效荧光团的激发偶极子和发射偶极子之间的夹角,均小于40°,且高钙低脂奶对应荧光团激发偶极子和发射偶极子之间的夹角略小于纯牛奶和高钙奶。实验还发现,在激发偏振片和发射偏振片取向平行时,高钙低脂奶的偏振荧光强度明显高于纯牛奶和高钙奶,与理论计算相符。Polarized three-dimensional fluorescence spectra of different kinds of milk were measured, including pure milk, milk with high calcium, and milk with high calcium but low fat produced by YiLi and MengNiu. It was found that the fluorescence in- tensity is stronger when excitation and emission polarizer orientation is parallel. The angle between excitation dipole and emission dipole of all kinds of milk equivalent chromophore was estimated by a simple model, indicating that they all less than 40°. The angle for milk with high calcium but low fat is less than that of pure milk and milk with high calcium. And in the experiment, the polarized fluorescence intensity of milk with high calcium but low fat is significantly greater than the others, which fitted with the model estimation.

关 键 词：光生物学 偏振荧光光谱 牛奶水溶液 偶极子 夹角 

分 类 号：O433.4[机械工程—光学工程]