疏肝和胃方对非糜烂性胃食管反流病模型大鼠内脏敏感性外周机制的影响  

Influence of Shugan Hewei Decoction on Periphery Esophageal Visceral Sensitivity in Non-erosive Gastroesophageal Reflux Disease Model Rats

作　　者：程艳梅[1] 张秀莲[1] 王高峰[2] 刘春芳[1] 朱生樑[1] 

机构地区：[1]上海中医药大学附属岳阳医院,上海200437 [2]昆山市中医院,江苏昆山215300

出　　处：《辽宁中医杂志》2012年第11期2147-2149,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：上海市教委脾胃病重点学科项目(JS0305);上海市卫生局课题(2010QJ023A);国家自然基金青年课题(81102567)

摘　　要：目的:观察疏肝和胃方对非糜烂性胃食管反流病(NERD)模型大鼠食道内脏高敏感外周机制的影响。方法:将60只SD大鼠随机分为空白对照组,生理盐水对照组,模型组,奥美拉唑组,疏肝和胃方组。除空白对照组外其余各组用腹腔注射鸡卵清蛋白(OVA)基础致敏联合食管酸灌注的方法复制NERD大鼠模型,连续给药14天后,观察各组大鼠食管组织超微结构变化、食管组织P物质及CGRP的含量变化。结果:与模型组比较,奥美拉唑组及疏肝和胃方组均能降低NERD模型大鼠P物质及CGRP表达(P<0.05),电镜显示NERD中药组食管组织核仁清晰,染色均匀;细胞间隙均匀。结论:疏肝和胃方可改善NERD大鼠的超微结构变化,下调食管组织中P物质及CGRP的表达,达到降低食道内脏敏感性的目的。Objective：To observe the effect and mechnism of Shugan Hewei Decoction on periphery esophageal visceral sensitivity in the non-erosive gastroesophageal reflux disease（NERD）model rats.Methods：Totally 60 Sprague-Dawley rats were randomized into a blank control group（normal group）,model group,Omeprazole Sodium group,Shugan Hewei Decoction group.Except for the normal group,the rats of all the other groups received basal ovalbumin-sensitization combined with intra-esophageal mucosal acid exposure for making NERD models.Then,the treatment was given for 14 days.The comparative observation was carried out on the changes of uhrastruetural characteristics and the expression of substance P（SP）and caltenin gene related peptide（CGRP）in esophagueal mucosa from SD rats.Results：Compared with the model group,the Omeprazole Sodium group and Shugan Hewei Decoction group also reduced the expressions of SP and CGRP in esophagueal mucosa from NERD model rats（P〈0.05）.Transmission electron microscope showed nucleolus clearness and coloretur well-distributed and interstitial space dilation well-distributed in esophagueal mucosa.Conclusion：Shugan Hewei Decoction plays a role in inhibiting esophageal visceral sensitivity by the way of improving the changes of uhrastruetural characteristics and down-regulating the expressions of SP and CGRP in esophagueal mucosa from rats.

关 键 词：非糜烂性胃食管反流病 内脏高敏感 疏肝和胃方 P物质 降钙素基因相关肽 

分 类 号：R655.4[医药卫生—外科学]