半边旗活性成分5F对C26结肠癌细胞cAMP水平的影响  

Effects of 5F on cAMP Levels in C26 Color Cancer Cells

作　　者：叶华[1,2] 吴琼[2,3] 郭蒙[2,3] 秦峥[1] 吕应年[2] 梁念慈[2] 郑学宝[1,2] 

机构地区：[1]南方医科大学,广东广州510515 [2]广东医学院广东天然药物研究与开发重点实验室,广东湛江524023 [3]广东医学院药理教研室,广东湛江524023

出　　处：《辽宁中医杂志》2012年第11期2150-2152,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(3987099);国家自然科学基金(30772701);湛江市科技攻关项目(2009C3104003);东莞市科技计划项目(2008108101036)

摘　　要：目的:探讨半边旗活性成分Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid(5F)对C26结肠癌细胞环磷酸腺苷(cAMP)水平的影响。方法:以不同时间、不同浓度5F处理C26细胞,放免法测定cAMP含量。磷酸二酯酶抑制剂3-Isobutyl-1-methylxanthine(IBMX)预先孵育后再与5F联合应用,放免法检测cAMP含量,MTT法检测C26细胞活力。结果:5F可以显著增加cAMP含量,具有时间和剂量依赖性;5F对cAMP含量的作用不受IBMX影响,IBMX可以增强5F对cAMP的促进作用和对C26细胞增殖的抑制作用。结论:5F可能通过激活腺苷酸环化酶(AC)增加C26细胞cAMP含量,这可能是5F抗肿瘤的作用机制之一,5F与IBMX联合应用具有协同效应。Objective：To investigate the effect of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid（5F）,a compound isolated from Pteris semipinnata L（PsL）,on cAMP levels in C26 color cancer cells.Methods：C26 cells were treated with 5F（0～40μg/mL）for different time periods.The cAMP levels were examined by radioimmunoassay.IBMX was used for pre-incubation and then combined with 5F,cAMP levels were measured.Viability was examined using a MTT method.Results：5F increased cAMP levels significantly,in a concentration and time dependent manner.The effect was not affected by IBMX.IBMX can enhance the role of 5F on cAMP levels and the inhibition of C26 cell proliferation.Conclusion：5F may increase the cAMP content of C26 cells by activating the adenylate cyclase（AC）,which may be one of the anti-tumor mechanisms of 5F.5F has a synergistic effect with IBMX.

关 键 词：半边旗 5F 结肠癌 CAMP 

分 类 号：R285.5[医药卫生—中药学]