成都地区婴幼儿腹泻诺如病毒感染研究  

Study on norovirus infection in diarrhea of children in Chengdu

作　　者：陈喜凯[1] 廖虹瑜[1] 叶倩[1] 胡鹏威[1] 曹霞[1] 裴晓方[1] 

机构地区：[1]四川大学公共卫生学院医学检验教研室,四川成都610041

出　　处：《现代预防医学》2012年第23期6123-6125,6129,共4页Modern Preventive Medicine

基　　金："艾滋病和病毒性肝炎等重大传染病防治科技"重大专项"传染病检测技术平台"项目;"云南省周边省市传染病病原谱流行规律研究"课题(2009ZX10004-212)

摘　　要：目的探讨四川成都地区婴幼儿腹泻中诺如病毒(Norovirus,NoV)的流行状况及基因特点。方法收集四川大学华西第二医院2009年9月～2011年5月的腹泻婴幼儿的粪便标本,采用逆转录-聚合酶链反应(RT-PCR)方法检测NoV核酸,通过测序进行分型和进化分析,统计分析NoV感染与性别、年龄、季节和临床症状特点的关系。结果 485例粪便标本中,Nov阳性60例,其中GⅠ簇5例(占8.3%),GⅡ簇54例(占90.0%),混合感染1例(占1.7%);12～24月龄儿童最易感染,未见性别和季节差异;呕吐与NoV感染存在中等强度的关联;24株GⅡ簇NoV中,GⅡ-4基因型占79.2%(19/24),GⅡ-3为16.7%(4/24),GⅡ-2型占4.1%(1/24);19株GⅡ-4中,有8株为2006a变异株,11株为2006b变异株;另外,4株NoV的重组分析显示并未发生重组现象。结论 2009年12月～2011年5月期间,NoV是引起成都地区婴幼儿非细菌性腹泻的主要病原体之一,GⅡ-4基因型(包括2006a和2006b变异株)为优势毒株,研究发现的一株GII-2型NoV国内未见报道,因此应对成都地区儿童的NoV感染进行长期监测和病原分析。OBJECTIVE To study the epidemiological and genetic characteristics of norovirus（NoV） in diarrhea of children in Chengdu.METHODS Collected the fecal samples from diarrhea children from the September of 2009 to the May of 2011 at West China Second University Hospital,Sichuan University,detected the nucleic acid of NoV by the method of RT-PCR,genotype and phylogenetically analyzed part of NoV stains by sequencing,and statistically analyzed the relation of NoV infection rates and genders,ages and seasons as well as the clinical symptoms.RESULTS Among 485 fecal samples,60 were identified positive including 5 cases of GⅠ cluster infection,54 cases of GⅡ cluster infection and one case of both infections.The children in the age of 12-24 months were more easily to get infected than other age groups while no significant difference was found in gender or season;there was a moderate correlation between vomit and NoV infection;Among 24 NoV strains,19（79.2%） were GⅡ-4,4（16.7%） were GⅡ-3 and the last one（4.1%） was GⅡ-2;in addition,19 strains of GⅡ-4 contained 8 strains of2006a and 11 strains of 2006b variants;recombinational analyses of 4 NoV strains were negative.CONCLUSION NoVis one of the most important pathogen causing children non-bacterial diarrhea in Chengdu from the September of 2009 to the May of 2011,and GⅡ-4 strains including 2006a and 2006b variants are the most prevalent strains,and that GⅡ-2 strain is a new genotype in China which has not reported before.So it is necessary to launch long-term monitoring and etiological analysis on the NoV infection of children in Chengdu.

关 键 词：诺如病毒 婴幼儿腹泻 基因分型 进化树分析 

分 类 号：R373.2[医药卫生—病原生物学]