硫酸镁对利多卡因中毒小鼠惊厥和死亡率的影响  

The effect of magnesium sulfate on the convulsion and mortality rate in lidocaine-poisoned mice

作　　者：邱有波[1] 杨拯[1] 袁梦郎[1] 

机构地区：[1]成都医学院,成都610081

出　　处：《现代预防医学》2012年第23期6266-6268,共3页Modern Preventive Medicine

摘　　要：目的应用盐酸利多卡因致小鼠中毒惊厥模型,探讨硫酸镁对利多卡因中毒小鼠惊厥和死亡率的影响。方法 210只昆明小鼠,体重18～25g,按照随机数字表随机分为5组:生理盐水对照组、50mg/kg硫酸镁组、100mg/kg硫酸镁组、200mg/kg硫酸镁组、400mg/kg硫酸镁组,每组42只,分别预先腹腔注射4种剂量的硫酸镁或等容积的生理盐水,15min后腹腔注射100mg/kg利多卡因,观察小鼠惊厥潜伏期、持续期、惊厥发生率、死亡时间和死亡率。结果①与生理盐水组相比,硫酸镁可延长利多卡因致惊厥的潜伏期,缩短利多卡因致惊厥的持续时间,延长利多卡因致惊厥的死亡时间。②硫酸镁可以降低利多卡因致惊厥的发生率,200和400mg/kg硫酸镁组与生理盐水组比较,利多卡因致惊厥的发生率差异具有统计学意义(P﹤0.05);可降低利多卡因致惊厥的死亡率,50、100、200mg/kg硫酸镁组与生理盐水比较,利多卡因致惊厥的死亡率差异具有统计学意义(P﹤0.01,P﹤0.05)。③硫酸镁可降低利多卡因致惊厥的强度,随着硫酸镁剂量的增加,利多卡因致惊厥的等级逐渐降低。结论在本实验条件下,硫酸镁可拮抗利多卡因的致惊厥作用,降低利多卡因的毒性。OBJECTIVE Using the model of seizure induced by lidocaine hydrochloride to investigate the effect of magnesium sulfate on the convulsions and mortality rate of mice with lidocaine poisoning. METHODS 210 Kunming mice weighing 18-25g were randomly divided into 5 groups： control group, 50mg / kg magnesium sulfate, 100mg / kg magnesium sulfate, 200mg / kg magnesium sulfate, 400mg / kg magnesium sulfate group, 42 mice in each group, and received intraperitoneal injections of magnesium sulfate of four different doses or the same volume of normal saline, respectively. 15 minutes later, all the mice received an intraperitoneal injection of 100mg / kg lidocaine hydrochloride, convulsion latency, duration, seizures incidence, death time and mortality in mice were observed. RESULTS ① Compared with control group, magnesium sulfate extended incubation period of lidocaine induced convulsions, shortened the duration of convulsions induced by lidocaine, extended the time of death of lidocaine induced convulsions. ② Magnesium sulfate could reduce the incidence of seizures induced by lidocaine, 200 and 400mg / kg magnesium group compared with the control group, the incidence of lidocaine induced convulsions had significant differences （P﹤0.05）; could reduce mortality of convulsions induced by lidocaine, 50, 100, 200 mg / kg magnesium group compared with the control group, lidocaine induced convulsions were significant differences in mortality （P﹤0.01, P﹤ 0.05）. ③ Magnesium sulfate could reduce the intensity convulsions caused by lidocaine. Increased with the dose of magnesium sulfate, the level of induced convulsions by lidocaine decreased. CONCLUSION In this experimental condition, magnesium sulfate could be antagonized by convulsive effects of lidocaine to reduce the toxicity of lidocaine.

关 键 词：硫酸镁 利多卡因 惊厥 死亡率 

分 类 号：R969[医药卫生—药理学]