病毒性胃肠炎的病原学检测  

Etiological analysis on viral gastroenteritis

作　　者：付建光[1] 祁贤[1] 吴斌[1] 洪镭[2] 张洪英[2] 

机构地区：[1]江苏省疾病预防控制中心,江苏南京210009 [2]南京市疾病预防控制中心

出　　处：《现代预防医学》2012年第23期6288-6289,6294,共3页Modern Preventive Medicine

基　　金：江苏省卫生厅预防医学研究课题(Y201024);南京市医学科技发展计划项目(ZKX09033)

摘　　要：目的了解某医院的不明原因胃肠炎病例的病原学特点,为科学预防可能的流行病提供科学依据。方法收集某医院2010年12月出现胃肠炎症状的10例患者的排泄物进行病原学检测,包括轮状病毒、诺如病毒、札幌病毒、星状病毒以及肠腺病毒5种肠道病毒的荧光定量PCR检测和测序分析。结果 10份粪便标本中,7例检测到诺如病毒核酸阳性,经测序分析为GII.4亚型,未检测到其他病毒,确定为该病毒感染所致。结论南京地区存在诺如病毒的威胁,导致该医院患者胃肠炎的病原是诺如病毒。与其他国家和地区一样,GII.4亚型为主要感染亚型,应加强相应的抗病毒健康教育宣传,尤其是可能导致传播的预防工作。OBJECTIVE To understand the main reasons for cases in one hospital with the gastroenteritis symptoms so as to provide scientific references for prevention and control of enteric infectious diseases. METHODS The stools of ten patients were collected and detected by real-time PCR and sequencing for the viral detection, including rotavirus, norovirus, Sapporo virus, astrovirus, adenovirus. RESULTS Norovirus was detected in 7 out of 10 stools, with same subtype of GII.4. The results confirmed the etiology wasviral infection. CONCLUSION There is the threat of norovirus in Nanjing. Norovirus, subtype GII.4, is the pathogen, resulting in the gastroenteritis in the hospital. It is the same subtype of norovirus as in other countries; anti-virus advocacy should be strengthened on health education and especially for prevention of infection.

关 键 词：医院 胃肠炎 病原学 诺如病毒 

分 类 号：R373.25[医药卫生—病原生物学]